CAJ | 학술논문

利用超高效液相色谱-质谱联用( UPLC-MS)技术结合主成分分析方法研究制川乌单煎液、制川乌与白芍、制川乌与防己共煎液在大鼠肠内菌中的代谢差异。采用SIMCA-P软件，以肠内菌代谢后乌头类生物碱的相对含量为变量进行主成分( PCA)分析。在主成分得分图中，制川乌单煎液与制川乌-白芍、制川乌-防己共煎液均可以明显区分，说明制川乌单煎液与制川乌-白芍、制川乌-防己共煎液的肠内菌生物转化存在显著差异。通过主成分分析载荷图及独立样本t检验，从制川乌-白芍组得到7种差异显著的标志物，从制川乌-防己组得到6种标志物，其中制川乌-白芍组有4种标志物经肠内菌代谢后含量高于制川乌组，而制川乌-防己组有1种化合物含量高于制川乌组，两组中其它标志物含量低于制川乌组。这些标志物可能是制川乌配伍前后药效差异的物质基础。
이용초고효액상색보-질보련용( UPLC-MS)기술결합주성분분석방법연구제천오단전액、제천오여백작、제천오여방기공전액재대서장내균중적대사차이。채용SIMCA-P연건，이장내균대사후오두류생물감적상대함량위변량진행주성분( PCA)분석。재주성분득분도중，제천오단전액여제천오-백작、제천오-방기공전액균가이명현구분，설명제천오단전액여제천오-백작、제천오-방기공전액적장내균생물전화존재현저차이。통과주성분분석재하도급독립양본t검험，종제천오-백작조득도7충차이현저적표지물，종제천오-방기조득도6충표지물，기중제천오-백작조유4충표지물경장내균대사후함량고우제천오조，이제천오-방기조유1충화합물함량고우제천오조，량조중기타표지물함량저우제천오조。저사표지물가능시제천오배오전후약효차이적물질기출。
Theultraperformanceliquidchromatographycoupledwithmassspectrometry(UPLC-MS)was used to investigate the metabolic difference of the decoction of Radix Aconiti Preparata ( RAP ) and its co-decoctions with Radix Paeoniae Alba ( RAP-RPA ) or Radix Stephaniae Tetrandrae ( RAP-RST ) in rat intestinal bacteria. The principal component analysis ( PCA) of the relative contents of Aconitum alkaloids after metabolism was performed by SIMCA-P software. The score plots of PCA could successfully distinguish the three groups of RAP, RAP-RPA and RAP-RST. The result indicated that the differences of biotransformation among the groups of PAP, RAP-RPA and RAP-RST were significant. With the loading plot and independent-samples T test, seven relevant markers with the significant differences were found in the group of RAP-RPA, six relevant markers were obtained in the group of RAP-RST. The relative content of four markers in RAP-RPA was higher than that in RAP, and one marker in RAP-RST was higher than that in RAP. The relative contents of other markers were all lower than that in RAP. These markers may be the effective substance for explaining the different effects of Radix Aconiti Preparata before and after combination with Radix Paeoniae Alba and Radix Stephaniae Tetrandrae.