CAJ | 학술논문

目的：探讨自身免疫性肝病（AILD）抗体谱检测在AILD诊断中的临床应用价值。方法采用线性免疫分析法检测不明病因肝功能异常、各种病毒性肝炎以及健康体检者（健康对照组）的血清标本，进行抗线粒体亚型-丙酮酸脱氢酶复合物（AMA/M2）、抗肝肾微粒体Ⅰ型抗体（LKM-1）、抗肝细胞胞浆Ⅰ型抗体（LC-1）、抗可溶性肝抗原/肝胰抗原抗体（SLA/LP）、糖蛋白210抗体（gp210）以及多核点抗原抗体（sp100）等6项指标的检验结果资料进行回顾分析。结果各组患者标本中的自身抗体总阳性检出率分别为自身免疫性肝炎（AIH）80．5％、原发性胆汁性肝硬化（PBC）92．3％、乙型肝炎病毒（HBV）17．2％、丙型肝炎病毒（HCV）16．7％、甲型肝炎病毒（HAV）22．2％、戊型肝炎病毒（HEV）14．3％，对照组仅为2．6％；AILD组的阳性检出率与其他各组间比较（P＜0．05）；AIH各亚型中AIH-Ⅰ的AMA/M2的阳性检出率与AIH-Ⅱ和AIH-Ⅲ比较（P＜0．05），AIH-Ⅱ的LKM-1的阳性检出率与 AIH-Ⅰ和 AIH-Ⅲ比较（P＜0．05），AIH-Ⅲ的SLA/LP的阳性检出率与 AIH-Ⅰ和 AIH-Ⅱ比较（P＜0．05）。结论 AILD抗体谱检测有助于AILD的早期诊断，可为该病的分型以及治疗提供有价值的实验室依据。
목적：탐토자신면역성간병（AILD）항체보검측재AILD진단중적림상응용개치。방법채용선성면역분석법검측불명병인간공능이상、각충병독성간염이급건강체검자（건강대조조）적혈청표본，진행항선립체아형-병동산탈경매복합물（AMA/M2）、항간신미립체Ⅰ형항체（LKM-1）、항간세포포장Ⅰ형항체（LC-1）、항가용성간항원/간이항원항체（SLA/LP）、당단백210항체（gp210）이급다핵점항원항체（sp100）등6항지표적검험결과자료진행회고분석。결과각조환자표본중적자신항체총양성검출솔분별위자신면역성간염（AIH）80．5％、원발성담즙성간경화（PBC）92．3％、을형간염병독（HBV）17．2％、병형간염병독（HCV）16．7％、갑형간염병독（HAV）22．2％、무형간염병독（HEV）14．3％，대조조부위2．6％；AILD조적양성검출솔여기타각조간비교（P＜0．05）；AIH각아형중AIH-Ⅰ적AMA/M2적양성검출솔여AIH-Ⅱ화AIH-Ⅲ비교（P＜0．05），AIH-Ⅱ적LKM-1적양성검출솔여 AIH-Ⅰ화 AIH-Ⅲ비교（P＜0．05），AIH-Ⅲ적SLA/LP적양성검출솔여 AIH-Ⅰ화 AIH-Ⅱ비교（P＜0．05）。결론 AILD항체보검측유조우AILD적조기진단，가위해병적분형이급치료제공유개치적실험실의거。
Objective To investigate the clinical application value of the autoimmune liver disease antibody spectrum detection in diagnosing autoimmune liver diseases (AILD) .Methods The linear immunoassay was adopted to detect the serum specimens in the patients with unknown etiology of liver function abnormalities ,various viral hep-atitis and healthy subjects with physical examination (control group) .The detection results of 6 indexes of anti-mito-chondrial subtype-pyruvate dehydrogenase complex (AMA/M2) ,liver kidney microsomal antibody type 1 (LKM-1 type) ,anti-liver cell cytoplasmic antibody-1(LC-1) ,type I anti soluble liver antigen /liver pancreas antibody (SLA/LP) ,glycoprotein 210 antibody(gp210)and multi nucleus antigen antibody (Sp100) were retrospectively analyzed .Re-sults The total positive detection rate of autoantibodies in various groups were 80 .5% in autoimmune hapatitis (AIH ) ,92 .3% in primary biliary cirrhosis (PBC ) ,17 .2% in hepatitis B virus (HBV ) ,16 .7% in hepatitis C virus (HCV) ,22 .2% in hepatitis A virus(HAV) and 14 .3% in hepatitis E virus(HEV) ,while which in the control group was only 2 .6% ;the positive detection rate in the autoimmune diseases group had statistical difference compared with other groups(P<0 .05);in the various AIH subtypes ,the positive detection rate of AMA/M2 had statistical differ-ence between AIH-Ⅰwith AIH-Ⅱ and AIH-Ⅲ(P<0 .05) ,the positive detection rate of LKM-1 had statistical differ-ence between AIH-Ⅱwith AIH-Ⅰand AIH-Ⅲ(P<0 .05) ,the positive testing rate of SLA/LP had statistical differ-ence between AIH-Ⅲ with AIH-Ⅰand AIH-Ⅱ(P<0 .05) .Conclusion The AILD antibody spectrum detection con-duces to early diagnosis of AILD and can provide the valuable laboratory basis for typing this disease and treatment .