CAJ | 학술논문

目的：研究白细胞介素（IL ）1β基因rs146219183位点在精神分裂症患者和健康对照组中的单核苷酸多态性，并测定精神分裂症患者一些生化指标和其单核苷酸多态性的关系。方法查询 rs146219183位点的基因序列，设计引物并合成，扩增后测序得到每个标本的等位基因和基因型。统计分析患者组和对照组等位基因，基因型是否存在统计学差异。用全自动生化分析仪检测精神分裂症患者血清中肝功能、肾功能及血脂等生化指标水平，统计分析不同等位基因、基因型患者之间差异。结果得到了100例精神分裂症患者和100例健康对照者的IL-1β基因rs146219183位点的单核苷酸多态性，且测定了精神分裂症患者的16项生化数据，经过统计学分析发现， rs146219183位点在精神分裂症患者和健康对照者中的A A、A T和T T 3种基因型频率上差异有统计学意义（χ2＝12．0547，P＝0．0024），A和T两种等位基因频率差异也有统计学意义（χ2＝8．1602，P＝0．0043），且AA、AT和TT 3种不同基因型患者的生化指标 CA、ALKP 和 GGT 差异也有统计学意义（P＜0．05）。结论 IL-1βrs146219183位点的单核苷酸多态性与精神分裂症有关，且不同基因型精神分裂症患者之间其生化指标CA、ALKP和GG T存在差异。
목적：연구백세포개소（IL ）1β기인rs146219183위점재정신분렬증환자화건강대조조중적단핵감산다태성，병측정정신분렬증환자일사생화지표화기단핵감산다태성적관계。방법사순 rs146219183위점적기인서렬，설계인물병합성，확증후측서득도매개표본적등위기인화기인형。통계분석환자조화대조조등위기인，기인형시부존재통계학차이。용전자동생화분석의검측정신분렬증환자혈청중간공능、신공능급혈지등생화지표수평，통계분석불동등위기인、기인형환자지간차이。결과득도료100례정신분렬증환자화100례건강대조자적IL-1β기인rs146219183위점적단핵감산다태성，차측정료정신분렬증환자적16항생화수거，경과통계학분석발현， rs146219183위점재정신분렬증환자화건강대조자중적A A、A T화T T 3충기인형빈솔상차이유통계학의의（χ2＝12．0547，P＝0．0024），A화T량충등위기인빈솔차이야유통계학의의（χ2＝8．1602，P＝0．0043），차AA、AT화TT 3충불동기인형환자적생화지표 CA、ALKP 화 GGT 차이야유통계학의의（P＜0．05）。결론 IL-1βrs146219183위점적단핵감산다태성여정신분렬증유관，차불동기인형정신분렬증환자지간기생화지표CA、ALKP화GG T존재차이。
Objective To study the single nucleotide polymorphisms of rs146219183 in IL-1βgene in schizo-phrenia patients and healthy people ,to detect some biochemistry indicators in schizophrenia patients and to investigate the relation between these biochemistry indicators and the single nucleotide polymorphisms .Methods Firstly the se-quence of rs146219183 was inquired ,then the primers were designed and synthesized .After amplification the allele and genotype of each sample were obtained by sequencing .Then total 100 schizophrenia patients and 100 healthy peo-ple were genotyped by PCR test .Some clinical biochemical indicators of the liver function ,renal function and blood lipids were detected by the fully automatic biochemical analyzer .The differences among the patients with different al-leles and genotypes were statistically analyzed .Results The single nucleotide polymorphisms of rs146219183 in IL-1βgene in 100 schizophrenia patients and 100 healthy people was obtained .16 biochemical indicators were detected . The statistical analysis found that the AA ,AT and TT3 genotype frequency of rs146219183 had statistical difference between the schizophrenia patients and healthy people (χ2 =12 .054 7 ,P=0 .002 4) .The A and T allele frequency al-so had statistical difference(χ2 =8 .160 2 ,P=0 .004 3) .Among the three groups of patients with different genotypes of AA ,AT and TT3 ,the differences in biochemical indicator CA ,ALKP and GGT also had statistical significance . Conclusion The single nucleotide polymorphisms of rs146219183 in IL-1βgene is associated with schizophrenia .The differences of biochemical indicator CA ,ALKP and GGT exist in schizophrenia patients with different genotypes .