CAJ | 학술논문

目的：了解强直性脊柱炎（AS）不同临床分期患者外周血淋巴细胞人类白细胞抗原B27（HLA-B27）表达量与疾病临床分期之间的关系。方法采用三色流式细胞学检测技术分析88例不同阶段AS患者及132例健康人的外周血淋巴细胞HLA-B27表达率及表达强度（MNX）。结果AS进展期患者外周血淋巴细胞HLA-B27表达率的中位数、P25、P75分别是95．34％、86．60％、97．39％，MNX的中位数、P25、P75分别为8．70、5．20、10．80；强直期患者外周血淋巴细胞HLA-B27表达率的中位数、P25、P75分别是94．51％、90．40％、97．83％，MNX的中位数、P25、P75分别为8．40、6．05、15．95。健康对照组的外周血淋巴细胞HLA-B27表达率的中位数、P25、P75分别是3．69％、1．03％、11．13％，MNX的中位数、P25、P75分别为1．50、1．30、1．90。Mann-WhitneyU检验显示，AS患者进展期与强直期的外周血淋巴细胞HLA-B27表达率及MNX均差异无统计学意义（P＞0．05）；进展期、强直期与健康对照组的外周血淋巴细胞HLA-B27表达率及MNX均差异有统计学意义（P＜0．05）。结论外周血淋巴细胞HLA-B27的表达率及MNX适合于AS诊断，对AS病情判断无辅助意义。
목적：료해강직성척주염（AS）불동림상분기환자외주혈림파세포인류백세포항원B27（HLA-B27）표체량여질병림상분기지간적관계。방법채용삼색류식세포학검측기술분석88례불동계단AS환자급132례건강인적외주혈림파세포HLA-B27표체솔급표체강도（MNX）。결과AS진전기환자외주혈림파세포HLA-B27표체솔적중위수、P25、P75분별시95．34％、86．60％、97．39％，MNX적중위수、P25、P75분별위8．70、5．20、10．80；강직기환자외주혈림파세포HLA-B27표체솔적중위수、P25、P75분별시94．51％、90．40％、97．83％，MNX적중위수、P25、P75분별위8．40、6．05、15．95。건강대조조적외주혈림파세포HLA-B27표체솔적중위수、P25、P75분별시3．69％、1．03％、11．13％，MNX적중위수、P25、P75분별위1．50、1．30、1．90。Mann-WhitneyU검험현시，AS환자진전기여강직기적외주혈림파세포HLA-B27표체솔급MNX균차이무통계학의의（P＞0．05）；진전기、강직기여건강대조조적외주혈림파세포HLA-B27표체솔급MNX균차이유통계학의의（P＜0．05）。결론외주혈림파세포HLA-B27적표체솔급MNX괄합우AS진단，대AS병정판단무보조의의。
Objective To study the relationship between the expression amount of peripheral blood lympho-cyte HLA-B27 with the clinical stages in the patients with different clinical stages of ankylosing spondylitis (AS) . Methods The expression rate and the expression intensity (MNX) of peripheral blood lymphocyte HLA-B27 were detected in 88 cases of different stages of AS and 132 healthy individuals by the three-color flow cytometry and the results were analyzed statistically .Results The median number ,P25 and P75 of peripheral blood lymphocyte HLA-B27 expression rate in the patients with progressive stage of AS were 95 .34% ,86 .60% and 97 .39% respectively , the median number ,P25 and P75 of MNX were 8 .70 ,5 .20 and 10 .80 respectively ;the median number ,P25 and P75 of peripheral blood lymphocyte HLA-B27 expression rate in the rigidity stage were 94 .51% ,90 .40% and 97 .83% respectively ,the median number ,P25and P75 of MNXwere 8 .40 ,6 .05 and 15 .95 respectively .The medi-an number ,P25 ,P75 of peripheral blood lymphocyte HLA-B27 expression rate in the healthy control group were 3 .69% ,1 .03% and 11 .13% ,respectively ,the median number ,P25 and P75 of MNX were 1 .50 ,1 .30 and 1 .90 respectively .The Mann-Whitney U test showed that the expression rate and MNX of peripheral blood lymphocyte HLA-B27 no statistically significant differences between the progressive stage and the rigidity stage in AS patients (P>0 .05);the differences in the expression rate and MNX of peripheral blood lymphocyte HLA-B27 among the progressive stage ,rigidity stage and the healthy control group had statistical significance .Conclusion The expres-sion rate and MNX of peripheral blood lymphocyte HLA-B27 are suitable for the diagnosis of AS and have no auxilia-ry significance in the judgment of AS condition .