CAJ | 학술논문

目的：探讨风湿病患者血清细胞因子检测的重要性，了解静脉血血清标本不同的放置时间对风湿性自身免疫疾病相关的细胞因子检测结果的影响。方法采集26例风湿病患者静脉血血清，分别于室温下放置0、1、4h后，通过液相悬浮芯片技术对其进行一系列细胞因子测定，并进行统计学分析。结果风湿病患者血清中白细胞介素（IL ）-10、IL-13、干扰素诱导蛋白10（IP-10）和干扰素 g （IFN-g ）等细胞因子水平与患者年龄相关（ P＜0．05）；血清中IL-10、IL-12p70、正常T细胞激活上调性表达因子（RANTES）、IP-10和巨噬细胞炎症蛋白1β（MIP-1β）等细胞因子水平与患者疾病类型相关（P＜0．05）；血清室温放置4 h内各标本中细胞因子水平差异无统计学意义（P＞0．05）。结论液相悬浮芯片技术可高效分析风湿病患者血清细胞因子谱，有助于疾病诊断和评估。风湿病患者血清细胞因子检测时限可延长至室温下4h，可以满足临床和基础研究的检测要求。
목적：탐토풍습병환자혈청세포인자검측적중요성，료해정맥혈혈청표본불동적방치시간대풍습성자신면역질병상관적세포인자검측결과적영향。방법채집26례풍습병환자정맥혈혈청，분별우실온하방치0、1、4h후，통과액상현부심편기술대기진행일계렬세포인자측정，병진행통계학분석。결과풍습병환자혈청중백세포개소（IL ）-10、IL-13、간우소유도단백10（IP-10）화간우소 g （IFN-g ）등세포인자수평여환자년령상관（ P＜0．05）；혈청중IL-10、IL-12p70、정상T세포격활상조성표체인자（RANTES）、IP-10화거서세포염증단백1β（MIP-1β）등세포인자수평여환자질병류형상관（P＜0．05）；혈청실온방치4 h내각표본중세포인자수평차이무통계학의의（P＞0．05）。결론액상현부심편기술가고효분석풍습병환자혈청세포인자보，유조우질병진단화평고。풍습병환자혈청세포인자검측시한가연장지실온하4h，가이만족림상화기출연구적검측요구。
Objective To investigate the importance of serum cytokine detection in rheumatic patients and to explore the effect of the different placing time on the detection results of rheumatic autoimmune diseases related cyto-kines .Methods Serum samples of venous blood were collected from 26 rheumatic patients and then placed at room temperature for 0 ,1 ,4 h .The cytokine concentrations were determined using the Bio-Plex protein array system .The detection results were statistically analyzed .Results The levels of IL-10 ,IL-13 ,IP-10 ,IFN-g were correlated with age(P<0 .05);the levels of IL-10 ,IL-12p70 ,RANTES ,IP-10 ,MIP-1β were correlated with disease types ;there was no significant differences in cytokine levels when the samples were placed at room temperature within 4 h .Conclusion The liquid suspension chip technology can measure serum cytokines spectrum efficiently ,which is benefit to the di-agnosis and estimation of disease .The detecting time limit of serum cytokines can be delayed to 4h at room tempera-ture ,which can satisfy the testing requirements for clinical and basic research .