CAJ | 학술논문

运用荧光猝灭的原理在pH 7．6的生理条件下,眼镜蛇毒蛋白与系列6-氨基-4-芳基-5-氰基-3-甲基-1-苯基吡啶[2,3-c]并吡唑化合物之间的相互作用被研究,并且筛选出6-氨基-4-对羟基苯基-5-氰基-3-甲基-1-苯基吡啶[2,3-c]并吡唑(I)进行了详细研究。猝灭常数KSV、结合常数K以及结合位点n被获得。该实验证明眼镜蛇毒蛋白与I之间生成了新的化合物,而这一过程是静态猝灭的过程,并且它们之间存在着一定的结合。
운용형광졸멸적원리재pH 7．6적생리조건하,안경사독단백여계렬6-안기-4-방기-5-청기-3-갑기-1-분기필정[2,3-c]병필서화합물지간적상호작용피연구,병차사선출6-안기-4-대간기분기-5-청기-3-갑기-1-분기필정[2,3-c]병필서(I)진행료상세연구。졸멸상수KSV、결합상수K이급결합위점n피획득。해실험증명안경사독단백여I지간생성료신적화합물,이저일과정시정태졸멸적과정,병차타문지간존재착일정적결합。
The interactions between the cobra venom protein and 6 -amino-4 -aryl-3 -methyl-1 -phenyl-1H-pyrazolo [3,4-b]pyridine-5-carbonitrile at pH 7. 6 were investigated by fluorescence quenching and 6-amino-4-(2-hydroxyphenyl) -3-methyl-1-phenyl-1H-pyrazolo[3,4-b]pyridine-5-carbonitrile (I) was studied in detail. The quenching constants KSV, binding constants K and sites n of I with the cobra venom protein were determined. The results indicated that both of interactions were static quenching procedures because of the formation of new compounds and there had some binding between them.