CAJ | 학술논문

目的：寻找一种适用于时间分辨荧光免疫试验测定17-羟孕酮（17-OHP）的室内质量控制方法。方法采用即刻法、L-J质控法和改进即刻法，统计20个质控数据，制作质控图。结果采用即刻法考核，前3次结果对后续质控结果影响较大，随后的结果会出现假在控。用改进即刻法考核可以有效地减少假失控和假在控结果的出现，较为适用于新生儿疾病筛查中时间分辨荧光免疫测定17-OHP的室内质量控制。结论时间分辨荧光免疫测定中手工操作步骤较多，操作细节都对实验结果有很大影响，需要规范实验操作严格执行。
목적：심조일충괄용우시간분변형광면역시험측정17-간잉동（17-OHP）적실내질량공제방법。방법채용즉각법、L-J질공법화개진즉각법，통계20개질공수거，제작질공도。결과채용즉각법고핵，전3차결과대후속질공결과영향교대，수후적결과회출현가재공。용개진즉각법고핵가이유효지감소가실공화가재공결과적출현，교위괄용우신생인질병사사중시간분변형광면역측정17-OHP적실내질량공제。결론시간분변형광면역측정중수공조작보취교다，조작세절도대실험결과유흔대영향，수요규범실험조작엄격집행。
Objective To find an internal quality controlling method for 17-OHP determination by time-resolved fluoroimmuno-assay .Methods 20 quality control data were collected .The data were analyzed by using L-J method ,instant method and improved instant method .Results were used for the construction of quality control charts .Result The first three quality control data had a great impact on the following judgments of internal quality controlling when instant method was used .The subsequent results might be false acceptance .Improved instant method could effectively reduce the situations of false run-away and false acceptance ,which was suitable for the internal quality control of 17-OHP determination by time-resolved fluoroimmunoassay in newborn screening . Conclusion There are many steps of manual operations in 17-OHP determination of time-resolved fluoroimmunoassay .The details of these operations have great impacts on the experimental results .Thus ,the operations of 17-OHP test should be specified and exe-cuted strictly according to requirement .