CAJ | 학술논문

目的：比较时间分辨荧光免疫分析技术（TRFIA）和ELISA法在检测 HBV血清学标志物的价值。方法分别采用TRFIA和ELISA法检测359例低浓度乙肝表面抗原（HBsAg）标本的 HBV血清学标志物，比较两种方法的检测结果的差异。结果 TRFIA法检测 HBV血清学标志物的各项结果阳性率均高于ELISA法，HBsAg、乙肝e抗原（HBeAg）和乙肝核心抗体（HB-cAb）阳性率结果差异有统计学意义（P＜0．05），而乙肝表面抗体（HBsAb）、乙肝e抗体（HBeAb）阳性率差异无统计学意义（P＞0．05）。结论与ELISA法相比，TRFIA在检测含有低浓度HBsAg标本时具有更高的灵敏度，具有良好的临床应用价值。
목적：비교시간분변형광면역분석기술（TRFIA）화ELISA법재검측 HBV혈청학표지물적개치。방법분별채용TRFIA화ELISA법검측359례저농도을간표면항원（HBsAg）표본적 HBV혈청학표지물，비교량충방법적검측결과적차이。결과 TRFIA법검측 HBV혈청학표지물적각항결과양성솔균고우ELISA법，HBsAg、을간e항원（HBeAg）화을간핵심항체（HB-cAb）양성솔결과차이유통계학의의（P＜0．05），이을간표면항체（HBsAb）、을간e항체（HBeAb）양성솔차이무통계학의의（P＞0．05）。결론여ELISA법상비，TRFIA재검측함유저농도HBsAg표본시구유경고적령민도，구유량호적림상응용개치。
Objective To compare the value of time-resolved fluorescence immunoassay (TRFIA) and ELISA for the detection of HBV serological markers .Methods To detect the HBV serological markers in 359 samples with low concentrations of HBsAg by TRFIA and ELISA respectively ,and to compare the results of the two methods .Results The levels of HBV serological markers detected by TRFIA were higher than those detected by ELISA .The positive rates of HBsAg ,HBeAg and HBcAb detected by TR-FIA and ELISA were significantly different(P<0 .05) ,while those of HBsAb and HBeAb were not(P>0 .05) .Conclusion Com-pared with ELISA ,TRFIA has higher sensitivity in the detection of low HBsAg concentration samples ,and it is valuable to be ap-plicated in clinical .