CAJ | 학술논문

根据H7亚型禽流感病毒的血凝素基因中的保守区域设计特异的LAMP引物。10倍倍比稀释试验表明了此方法的高灵敏性，最低可检测到100 fg的RNA。对H1、H3、H4、H5、H6、H9亚型A型流感病毒和NDV进行H7－RT－LAMP检测，并无交叉反应，说明了此方法的高特异性。利用80份临床样品对所建立的H7－RT－LAMP方法进行评价，鉴定结果和病毒分离方法一致，说明此方法的可行性。建立的H7－RT－LAMP方法简单、快速、灵敏、特异，是H7亚型禽流感病毒的一种高效检测方法，适合在基层进行H7亚型禽流感病毒的早期筛检。
근거H7아형금류감병독적혈응소기인중적보수구역설계특이적LAMP인물。10배배비희석시험표명료차방법적고령민성，최저가검측도100 fg적RNA。대H1、H3、H4、H5、H6、H9아형A형류감병독화NDV진행H7－RT－LAMP검측，병무교차반응，설명료차방법적고특이성。이용80빈림상양품대소건립적H7－RT－LAMP방법진행평개，감정결과화병독분리방법일치，설명차방법적가행성。건립적H7－RT－LAMP방법간단、쾌속、령민、특이，시H7아형금류감병독적일충고효검측방법，괄합재기층진행H7아형금류감병독적조기사검。
The RT-LAMP primers were designed to target the HA gene of H7 subtype of avian influ?enza viruses. Results of 10?fold dilution series assays showed that analysis of RT-LAMP products by the real?time fluorescence method was highly sensitive, and that the analytic sensitivities of this method was 100 fg. Using a pool of RNAs extracted from influenza viruses corresponding to H1, H3, H4, H5, H6 and H9 HA subtypes and NDV, the RT-LAMP system was confirmed to amplify only H7 AIV RNA. The performance characteristics of the RT-LAMP assay were evaluated with 80 clini?cal specimens collected from suspected poultry and wild birds, the results of which were consistent with virus isolation and demonstrated the feasibility of the method. Overall, the RT-LAMP assay es?tablished in this study is simple, rapid, sensitive and specific, which can be used as a reliable method for early diagnosis of the influenza A( H7N9) virus infection, especially in underequipped laboratories.