CAJ | 학술논문

为建立一种简单快速、高效制备人参皂苷20（ S）?Rg3、20（ R）?Rg3、Rg5、Rk1的方法。利用大孔吸附树脂和硅胶柱层析对样品进行预处理，通过反相高效制备液相色谱，从红参的甲醇提取物中快速分离得到目标产物人参皂苷20（S）?Rg3、20（R）?Rg3、Rg5、Rk1，经检测，4种化合物的纯度均＞98％。色谱柱为Polaris C18（250 mm ×4?6 mm，10μm），以V（乙腈）∶ V（水）＝46∶54为流动相，恒梯度洗脱，流速4?0 mL／min，柱温室温，检测波长为203 nm，在此色谱条件下，它们得率分别为0?012％、0?013％、0?012％、0?013％。该方法操作简便，可重复进样，适用于制备高纯度稀有人参皂苷20（ S）?Rg3、20（ R）?Rg3、Rg5、Rk1。
위건립일충간단쾌속、고효제비인삼조감20（ S）?Rg3、20（ R）?Rg3、Rg5、Rk1적방법。이용대공흡부수지화규효주층석대양품진행예처리，통과반상고효제비액상색보，종홍삼적갑순제취물중쾌속분리득도목표산물인삼조감20（S）?Rg3、20（R）?Rg3、Rg5、Rk1，경검측，4충화합물적순도균＞98％。색보주위Polaris C18（250 mm ×4?6 mm，10μm），이V（을정）∶ V（수）＝46∶54위류동상，항제도세탈，류속4?0 mL／min，주온실온，검측파장위203 nm，재차색보조건하，타문득솔분별위0?012％、0?013％、0?012％、0?013％。해방법조작간편，가중복진양，괄용우제비고순도희유인삼조감20（ S）?Rg3、20（ R）?Rg3、Rg5、Rk1。
A simple, fast and efficient method for the preparation of ginsenosides 20( S)?Rg3, 20 ( R)?Rg3 , Rg5 and Rk1 was constructed. Using macroporous absorption resin and silica gel column chromatography, the samples were pretreated. Through RP?HPLC, target components 20(S)?Rg3, 20( R)?Rg3 , Rg5 and Rk1 were rapidly separated from the methanol extracts of red ginseng with the purity over 98%. The separation was performed on a Polaris C18 (250 mm × 4?6 mm, 10 μm) at the room temperature by mobile phase of CH3CN ∶ H2O (46 ∶ 54) at a flow rate of 4?0 mL/min and the detection wavelength was 203 nm. Under these conditions, the yield rates of four ginsenosides were 0?012%, 0?013%, 0?012% and 0?013%, respectively. This method is effective and reliable for the preparation of 20( S)?Rg3 , 20( R)?Rg3 ,Rg5 and Rk1 from red ginseng.