CAJ | 학술논문

通过简并引物克隆得到了桦褐孔菌及同属真菌共11个菌株的漆酶基因片段，并对其结构与同源性进行了分析。结果表明：11个目的扩增产物均含有2个内含子，且内含子位置保守，推导的氨基酸序列中均包含有保守的铜离子结合位点；DNA序列与氨基酸序列的相似度都颇高，基于2类序列构建的NJ系统进化树均能将除俄罗斯采集的桦褐孔菌菌株外归为1组，该漆酶基因片段可用于鉴定桦褐孔菌。
통과간병인물극륭득도료화갈공균급동속진균공11개균주적칠매기인편단，병대기결구여동원성진행료분석。결과표명：11개목적확증산물균함유2개내함자，차내함자위치보수，추도적안기산서렬중균포함유보수적동리자결합위점；DNA서렬여안기산서렬적상사도도파고，기우2류서렬구건적NJ계통진화수균능장제아라사채집적화갈공균균주외귀위1조，해칠매기인편단가용우감정화갈공균。
Laccase gene fragments of 11 strains of Inonotus obliquus and several other species in the same genus were cloned by using degenerate primers, and its structure and homology were also ana?lyzed. The results indicated that two introns, the positions of which were highly conserved, were contained in all 11 PCR-amplified products, and conserved copper?binding sites ( His) were shown in the alignment of amino acid sequences. High similarities were reveled in both DNA and amino acid sequences, and the NJ trees constructed on the basis of the two sequences displayed that all I. obliquus strains expect Chaga were grouped together, suggesting this laccase gene fragment could be applied in species identification of I. obliquus.