CAJ | 학술논문

菌体细胞内存在的与细胞结构和功能不相关的物质可以通过多物质抗性泵出系统运输排出细胞外。某些有机溶剂耐受菌可以通过泵出系统将细胞膜上及细胞内的有机溶剂分子泵出到细胞外。这些有机溶剂耐受菌通常表现出对抗生素耐受性的提高，通过测定细菌细胞抗生素耐受性可以测定其细胞内是否存在有机溶剂外排泵。为确定有机溶剂耐受菌株Bacillus cereus UD－8细胞内是否存在有机溶剂外排泵系统，分别研究了Bacillus cereus UD－8的有机溶剂耐受性；四环素、氯霉素、氨苄青霉素、林可霉素的抗药性及4种抗生素对Bacillus cereus UD－8的最小抑菌浓度。初步断定蜡状芽孢杆菌Bacillus cereus UD－8可能具有有机溶剂泵出机制。
균체세포내존재적여세포결구화공능불상관적물질가이통과다물질항성빙출계통운수배출세포외。모사유궤용제내수균가이통과빙출계통장세포막상급세포내적유궤용제분자빙출도세포외。저사유궤용제내수균통상표현출대항생소내수성적제고，통과측정세균세포항생소내수성가이측정기세포내시부존재유궤용제외배빙。위학정유궤용제내수균주Bacillus cereus UD－8세포내시부존재유궤용제외배빙계통，분별연구료Bacillus cereus UD－8적유궤용제내수성；사배소、록매소、안변청매소、림가매소적항약성급4충항생소대Bacillus cereus UD－8적최소억균농도。초보단정사상아포간균Bacillus cereus UD－8가능구유유궤용제빙출궤제。
The substances which are structurally and functionally irrelevant to bacterial cells can be discharged through multi?material resistant efflux pump. Certain organic solvent tolerant bacteria re?move intracellular and cytomembrane?mounted organic solvent molecules through the efflux pump. Signs of resistance to antibiotics are generally shown by these solvent tolerant bacteria, therefore, by measuring the bacteria cells′resistance to antibiotics, existence of intracellular organic solvent efflux pump can be confirmed. Experiments on MIC ( minimum inhibitory concentration) and tolerance of Bacillus cereus UD-8 to tetracycline, chloramphenicol, ampicillin and lincomycin were taken during the investigation to preliminarily determine mechanism for organic solvent efflux pump of Bacillus ce?reus UD-8.