CAJ | 학술논문

To explore counter-defense response mechanism of pine caterpillar ( Dendrolinus punctatus tabulaeformis Tsai et Liu) to the induced insect-resistance of Chinese pine (Pinus tabulaeformis),the Chinese pine was fed by different amount pine caterpillars to obtain induced insect-resistance in Pingquan of Hebei Province,and main detoxifying enzymes, protective enzymes and digestive enzymes of pine caterpillars fed on the Chinese pine leaf were determined. The results showed that most of enzymes in the pine caterpillars changed obviously after feeding various induced resistant pine leaves. Carboxylesterase,glutathione-transferase,superoxide dismutase,peroxidase and protease activity of the lavae increased significantly with the increasing feeding stimulation. Acetylcholinesterase,lipase and amylase activity of the lavae had some change but no obviously linear variation,and acetylcholinesterase activity had no change. The results indicated that carboxyl esterase,glutathione-s-transferees,superoxide dismutase,peroxidase and protease were important enzymes with that pine caterpillar responded to the pine defense. Within a certain range,the adaptive enzymes activity in the pine caterpillar increased as the increasing of the action strength and time of the pine induced defensive products to pine caterpillar.