CAJ | 학술논문

通过扫描电镜观察蚜科专化菌暗孢耳霉对竹梢凸唇斑蚜的侵染特征。孢子附着在蚜虫体表后可迅速萌发直接侵入虫体，24 h后即有蚜虫感病死亡。在寄主死亡6 h内，假囊状体先突破体壁，分生孢子梗随后出现，后者可通过产孢启动新一轮的侵染循环。毒力生物测定结果显示，暗孢耳霉对竹梢凸唇斑蚜具有高毒力。在高剂量接种浓度(192.6±20.3)个孢子· mm －2时，92.8%的接种蚜虫5天内死亡。经时间－剂量－死亡率模型分析，第5天的半致死浓度低至51.6个孢子· mm －2 ，半致死时间在接种浓度100个孢子· mm －2时仅为2.3 天。
통과소묘전경관찰아과전화균암포이매대죽소철진반아적침염특정。포자부착재아충체표후가신속맹발직접침입충체，24 h후즉유아충감병사망。재기주사망6 h내，가낭상체선돌파체벽，분생포자경수후출현，후자가통과산포계동신일륜적침염순배。독력생물측정결과현시，암포이매대죽소철진반아구유고독력。재고제량접충농도(192.6±20.3)개포자· mm －2시，92.8%적접충아충5천내사망。경시간－제량－사망솔모형분석，제5천적반치사농도저지51.6개포자· mm －2 ，반치사시간재접충농도100개포자· mm －2시부위2.3 천。
Bamboo forests are multi-function resources,which frequently suffer from bamboo aphid infestation. This study aimed to estimate the potential of the aphid-specific pathogen Conidiobolus obscurus on bamboo aphid biocontrol. Tthe fungal infection features on Takecallis taiwanus were observed by scanning electronic microscopy. Conidia adherent to the aphid cuticle could germinate rapidly to invade into host body. 24 h after inoculation,first cadavers appeared. Cystidia penetrated the cuticle in 6 h after host death,and followed by conidiophores,which sporulate for renewing next infection cycle. The result of multi-concentration bioassay showed that C. obscurus had high infectivity to T. taiwanus. After inoculated at high conidial concentration of ( 192. 6 ± 20. 3 ) conidia· mm - 2 ,92. 8% aphids died in 5 days. The bioassay data were well fitted to the time-concentration-mortality model, and the lowest value of the median lethal concentration was computed as 51. 6 conidia· mm - 2 on day 5. Meanwhile,the median lethal time was computed as 2. 3 days under the inoculation of 100 conidia· mm - 2 . All the results presented the promising potential of C. obscurus on bamboo aphid pest control.