CAJ | 학술논문

力生长因子(MGF,mechano-growth fac-tor)是由 Yang 等发现并命名,是细胞对力刺激响应的产物,对人骨髓间充质干细胞(hMSC,human mesen-chymal stem cells)有重要的调控作用。利用静电纺丝技术(electrospinning)及相分离法制备了基于聚已内酯(PCL,polycaprolactone)的含 MGF 的葡聚糖纳米微球的纤维支架,间接性地保护了 MGF 活性,采用扫描电镜和透射电镜对微球和纤维支架进行了基础表征。同时,进一步采用激光共聚焦显微镜和酶标仪分别考察了纳米微球中释放出来的 MGF 对细胞的作用。研究结果表明含 MGF 的纳米微球支架能够显著促进 hMSC 增殖和迁移。此静电纺丝纤维支架能够较好地保护 MGF 的活性,有望在组织工程领域得到应用。
력생장인자(MGF,mechano-growth fac-tor)시유 Yang 등발현병명명,시세포대력자격향응적산물,대인골수간충질간세포(hMSC,human mesen-chymal stem cells)유중요적조공작용。이용정전방사기술(electrospinning)급상분리법제비료기우취이내지(PCL,polycaprolactone)적함 MGF 적포취당납미미구적섬유지가,간접성지보호료 MGF 활성,채용소묘전경화투사전경대미구화섬유지가진행료기출표정。동시,진일보채용격광공취초현미경화매표의분별고찰료납미미구중석방출래적 MGF 대세포적작용。연구결과표명함 MGF 적납미미구지가능구현저촉진 hMSC 증식화천이。차정전방사섬유지가능구교호지보호 MGF 적활성,유망재조직공정영역득도응용。
Mechano-growth factor (MGF)was found and named by Yang,generated by cells in response to me-chanical stimulation,playing a key role in regulating mesenchymal stem cell (MSC)function.In this study, pre-formulated dextran glassy nanoparticles (DGNs)loaded with MGF through freezing-induced phase separa-tion were electrospun into a polycaprolactone (PCL)copolymer fiber to secure the bioactivity of MGF,which was characterized by field emission scanning electron microscopy (FESEM)and transmission electron microsco-py (TEM),and then bioactivity retention was certificated by the change of hMSC behaviour with confocal laser scanning microscopy and spectrophotometric microplate reader.The enhanced cell proliferation and migration show that the MGF-DGNs-loaded PCL fibrous membrane can release MGF sustainably and secure the bioactivi-ty of MGF better than other PCL fibrous membranes,which may help find MGF-based fibrous membranes ap-plication of MSC in tissue engineering.