CAJ | 학술논문

采用酶学方法测定了不同性腺发育时期(Ⅱ~Ⅴ期)小体鲟Acipenser ruthenus Linnaeus雌性亲鱼胃、肠道和幽门盲囊中蛋白酶、淀粉酶、脂肪酶的活性,并分析了3种酶活性随性腺发育时期的变化规律。结果表明：小体鲟雌性亲鱼5种消化器官中的蛋白酶活性以性腺发育Ⅱ期、Ⅲ期较高,淀粉酶活性以性腺发育Ⅴ期较高,脂肪酶活性以性腺发育Ⅴ期、Ⅱ期较高,3种酶活性均以性腺发育Ⅳ期较低；各性腺发育期,雌性亲鱼的蛋白酶活性均以幽门盲囊、后肠中较高,其次为胃、中肠,淀粉酶活性以中肠中最高,其次为胃、幽门盲囊、前肠、后肠,脂肪酶活性均以幽门盲囊中最高,其次为中肠、前肠、胃、后肠。研究表明,小体鲟雌性亲鱼在性腺发育Ⅱ期、Ⅲ期对食物中蛋白质和碳水化合物需求较高,而性腺发育周期中对脂类物质的需求Ⅴ期最高,其次是Ⅱ期,总体来说,小体鲟亲鱼在整个性腺发育时期对脂肪的需求量较高。
채용매학방법측정료불동성선발육시기(Ⅱ~Ⅴ기)소체심Acipenser ruthenus Linnaeus자성친어위、장도화유문맹낭중단백매、정분매、지방매적활성,병분석료3충매활성수성선발육시기적변화규률。결과표명：소체심자성친어5충소화기관중적단백매활성이성선발육Ⅱ기、Ⅲ기교고,정분매활성이성선발육Ⅴ기교고,지방매활성이성선발육Ⅴ기、Ⅱ기교고,3충매활성균이성선발육Ⅳ기교저；각성선발육기,자성친어적단백매활성균이유문맹낭、후장중교고,기차위위、중장,정분매활성이중장중최고,기차위위、유문맹낭、전장、후장,지방매활성균이유문맹낭중최고,기차위중장、전장、위、후장。연구표명,소체심자성친어재성선발육Ⅱ기、Ⅲ기대식물중단백질화탄수화합물수구교고,이성선발육주기중대지류물질적수구Ⅴ기최고,기차시Ⅱ기,총체래설,소체심친어재정개성선발육시기대지방적수구량교고。
Activities of protease, amylase and lipase were analyzed and compared in stomach, intestine and pyloric caecum in female sterlet Acipenser ruthenus broodstock with different developmental phases (Ⅱ-Ⅴ) . The results showed that the maximal protease activity was observed in the broodstock withⅡphase ovary, and that higher pro-tease activity in the broodstock withⅢphase ovary. The amylase activity was found to be higher in the broodstock with Ⅴphase ovary, and the lipase activity to be higher in broodstock withⅤandⅡphase ovaries. The minimal activities of protease, amylase and lipase were observed in the broodstock with Ⅳ phase ovary, and in the brood-stock with different gonadal developmental phases higher protease activity was observed in pyloric caecum and hind-gut, followed by in the stomach and midgut. The maximal amylase activity, however, was found in hindgut, fol-lowed by in the stomach, pyloric caecum, foregut and hindgut. The maximal lipase activity was observed in the py-loric caecum, and higher lipase activity was found in the intestine and stomach. The findings indicated that the sterlet broodstock with Ⅱ and Ⅲ phase ovary required more dietary protein and carbohydrate, while the sterlet broodstock with Ⅴor Ⅱphase ovary needed more lipid. During the whole gonadal development, the sterlet brood-stock required more dietary lipid.