大连海洋大学学报
大連海洋大學學報
대련해양대학학보
JOURNAL OF DALIAN FISHERIES UNIVERSITY
2014年
5期
469-475
,共7页
李莉萍%王瑞%黄婷%梁万文%雷爱莹%李健%黄维义%唐佳有%施金谷%甘西%陈明
李莉萍%王瑞%黃婷%樑萬文%雷愛瑩%李健%黃維義%唐佳有%施金穀%甘西%陳明
리리평%왕서%황정%량만문%뢰애형%리건%황유의%당가유%시금곡%감서%진명
罗非鱼%无乳链球菌%血清型
囉非魚%無乳鏈毬菌%血清型
라비어%무유련구균%혈청형
Oreochromis niloticus%Streptococcus agalactiae%serotype
采用PCR血清型鉴定方法,对2007-2012年从广西、广东、海南、福建和云南等地养殖的罗非鱼Oreochromis niloticus中分离获得的168株无乳链球菌Streptococcus agalactiae流行菌株的血清型进行分析。结果表明：有159株为Ⅰa血清型,6株为Ⅰb血清型,3株为Ⅲ血清型。各区域流行菌株血清型也存在差异：从广西自治区7个地区50个养殖场的罗非鱼中分离的61株无乳链球菌中,有54株为Ⅰa型,4株为Ⅰb型,3株为Ⅲ型；从广东省10个地区59个养殖场的罗非鱼中分离的64株无乳链球菌中,有63株为Ⅰa型,1株为Ⅰb型；从海南省5个地区26个养殖场的罗非鱼中分离的33株无乳链球菌中,有32株为Ⅰa型,1株为Ⅰb型；从福建省漳州6个养殖场的罗非鱼中分离的6株和从云南省文山4个养殖场的罗非鱼中分离的4株均为Ⅰa型。研究表明,2007-2012年中国罗非鱼链球菌病流行菌株血清型存在Ⅰa、Ⅰb和Ⅲ3种血清型,Ⅰa型为主要血清型,研究结果可为准确分析中国罗非鱼链球菌病流行规律和特点提供数据,为该病防治及疫苗候选菌株筛选提供理论基础。
採用PCR血清型鑒定方法,對2007-2012年從廣西、廣東、海南、福建和雲南等地養殖的囉非魚Oreochromis niloticus中分離穫得的168株無乳鏈毬菌Streptococcus agalactiae流行菌株的血清型進行分析。結果錶明：有159株為Ⅰa血清型,6株為Ⅰb血清型,3株為Ⅲ血清型。各區域流行菌株血清型也存在差異：從廣西自治區7箇地區50箇養殖場的囉非魚中分離的61株無乳鏈毬菌中,有54株為Ⅰa型,4株為Ⅰb型,3株為Ⅲ型；從廣東省10箇地區59箇養殖場的囉非魚中分離的64株無乳鏈毬菌中,有63株為Ⅰa型,1株為Ⅰb型；從海南省5箇地區26箇養殖場的囉非魚中分離的33株無乳鏈毬菌中,有32株為Ⅰa型,1株為Ⅰb型；從福建省漳州6箇養殖場的囉非魚中分離的6株和從雲南省文山4箇養殖場的囉非魚中分離的4株均為Ⅰa型。研究錶明,2007-2012年中國囉非魚鏈毬菌病流行菌株血清型存在Ⅰa、Ⅰb和Ⅲ3種血清型,Ⅰa型為主要血清型,研究結果可為準確分析中國囉非魚鏈毬菌病流行規律和特點提供數據,為該病防治及疫苗候選菌株篩選提供理論基礎。
채용PCR혈청형감정방법,대2007-2012년종엄서、엄동、해남、복건화운남등지양식적라비어Oreochromis niloticus중분리획득적168주무유련구균Streptococcus agalactiae류행균주적혈청형진행분석。결과표명：유159주위Ⅰa혈청형,6주위Ⅰb혈청형,3주위Ⅲ혈청형。각구역류행균주혈청형야존재차이：종엄서자치구7개지구50개양식장적라비어중분리적61주무유련구균중,유54주위Ⅰa형,4주위Ⅰb형,3주위Ⅲ형；종광동성10개지구59개양식장적라비어중분리적64주무유련구균중,유63주위Ⅰa형,1주위Ⅰb형；종해남성5개지구26개양식장적라비어중분리적33주무유련구균중,유32주위Ⅰa형,1주위Ⅰb형；종복건성장주6개양식장적라비어중분리적6주화종운남성문산4개양식장적라비어중분리적4주균위Ⅰa형。연구표명,2007-2012년중국라비어련구균병류행균주혈청형존재Ⅰa、Ⅰb화Ⅲ3충혈청형,Ⅰa형위주요혈청형,연구결과가위준학분석중국라비어련구균병류행규률화특점제공수거,위해병방치급역묘후선균주사선제공이론기출。
PCR typing assay was used to evaluate serotype of 168 Streptococcus agalactiae prevalent strains isolated from Nile tilapia Oreochromis niloticus in Guangxi,Guangdong,Hainan,Fujian and Yunnan provinces in China from 2007 to 2012. There were 159 strains ofⅠa,6 strains ofⅠb, and 3 strains of Ⅲ, with differences in serotypes of isolates from different regions. In the 61 strains isolated from 50 farms in 7 regions in Guangxi,54 strains wereⅠa, 4 strains Ⅰb, and 3 strains Ⅲ. There were 64 strains isolated from 59 farms in 10 regions in Guangdong, with 63 strains of Ⅰa,1 strain of Ⅰb. Thirty-three strains were isolated from 26 farms in 5 regions in Hannan, including 32 strains of Ⅰa, and 1 strains ofⅠb. Four strains ofⅠa were isolated from 6 farms in Zhangzhou in Fujian and Wenshan in Yunnan. The 168 S. agalactiae prevalent strains isolated from tilapia in China during 2007-2012 were identified as 3 serotypes (Ⅰa,Ⅰb andⅢ) ,with the predominant serotypeⅠa. The findings provide data for analy-sis of the rules and characteristics of tilapia S. agalactiae epidemic, and therefore provide a theoretical basis for pre-vention and control of the tilapia streptococcicosis and for screening vaccine candidate strains of S. agalactiae in ti-lapia.
