CAJ | 학술논문

利用核糖体DNA第一内转录间隔区(Internal transcribed spacer 1, ITS1)序列,对中国大连(CD)、朝鲜罗津( KN)和俄罗斯海参崴( RV)仿刺参Apostichopus japonicus 3个群体的遗传结构进行分析。结果表明：经PCR扩增、克隆测序,获得长度为517~519 bp、524 bp两种类型的ITS1核苷酸序列,平均G+C含量(64.5%)显著高于A+T含量(35.5%)；在仿刺参30个个体61条序列中共检测到49个变异位点,多态位点比例为9.33%,其中有4个简约信息位点,共有40种基因型,群体共享基因型为2个；遗传多样性分析表明,3个群体的遗传多样性丰富；分子方差分析显示,88.65%的变异来自于群体内部,群体间遗传分化较弱或中度分化；根据群体间遗传距离进行的聚类分析发现, KN群体与RV群体的亲缘关系较近, CD群体与KN、 RV群体的亲缘关系较远。
이용핵당체DNA제일내전록간격구(Internal transcribed spacer 1, ITS1)서렬,대중국대련(CD)、조선라진( KN)화아라사해삼외( RV)방자삼Apostichopus japonicus 3개군체적유전결구진행분석。결과표명：경PCR확증、극륭측서,획득장도위517~519 bp、524 bp량충류형적ITS1핵감산서렬,평균G+C함량(64.5%)현저고우A+T함량(35.5%)；재방자삼30개개체61조서렬중공검측도49개변이위점,다태위점비례위9.33%,기중유4개간약신식위점,공유40충기인형,군체공향기인형위2개；유전다양성분석표명,3개군체적유전다양성봉부；분자방차분석현시,88.65%적변이래자우군체내부,군체간유전분화교약혹중도분화；근거군체간유전거리진행적취류분석발현, KN군체여RV군체적친연관계교근, CD군체여KN、 RV군체적친연관계교원。
The genetic diversity and genetic differentiation were studied in three wild populations of sea cucumber Apostichopus japonicus from Dalian China ( CD) ,Rajin North Korea ( KN) and Vladivostok Russia( RV) by internal transcribed spacer 1(ITS1). Two different length of ITS1 sequence (517-519 bp and 524 bp) were obtained via cloning and sequencing of PCR products, with higher mean contents of G+C ( 64. 5%) than those of A+T (35. 5%). There were 49 variation sites from 61 sequences in the 3 populations, with polymorphic sites of 9. 33%, and 4 parisimony informative among 40 genotypes including 2 shared genotypes. Genetic diversity parame-ters showed high genetic diversity among populations. The fixation indices (FST) of analysis of molecular variance (AMOVA) within populations was found to be 88. 65%,indicating that the three populations had weak or only moderately genetic differentiation. The molecular phylogenetic tree constructed with NJ method based on genetic distance revealed that there was closer linkage between KN population and RV population than between them and CD population.