CAJ | 학술논문

以从日本引进的Eisenia bicyclis雌、雄配子体为材料,研究了不同温度(10、15、20℃)和光照强度[20、40、60μmol/(m2·s)]对雌、雄配子体生长发育和幼孢子体生长的影响。结果表明：雌、雄配子体在温度为20℃时生长最快,15℃时次之,10℃时最慢,在温度为10℃时随光照强度的升高其生长速度加快,但随温度的升高不同光照组间的生长差异逐渐缩小；15℃和20℃是雌、雄配子体发育成熟的适宜温度,培养12 d后配子体的成熟率可达到95%~100%,而且高光照条件下的成熟率明显优于低光照下；幼孢子体生长的适宜温度为20℃,在各光照条件下的生长速度明显快于温度为15、10℃组；温度为10℃时,光照强度越高幼孢子体生长越快,温度为15、20℃时,幼孢子体在光照强度为20μmol/( m2·s)时的生长速度明显慢于40、60μmol/( m2·s)组,而40μmol/( m2·s)组与60μmol/( m2·s)组间生长差异不明显；将雌、雄配子体打碎进行克隆苗繁育,在室内培养至体长为800μm以上的幼孢子体,将其移至海区暂养,1个月后可生长成2 cm左右的幼体。
이종일본인진적Eisenia bicyclis자、웅배자체위재료,연구료불동온도(10、15、20℃)화광조강도[20、40、60μmol/(m2·s)]대자、웅배자체생장발육화유포자체생장적영향。결과표명：자、웅배자체재온도위20℃시생장최쾌,15℃시차지,10℃시최만,재온도위10℃시수광조강도적승고기생장속도가쾌,단수온도적승고불동광조조간적생장차이축점축소；15℃화20℃시자、웅배자체발육성숙적괄의온도,배양12 d후배자체적성숙솔가체도95%~100%,이차고광조조건하적성숙솔명현우우저광조하；유포자체생장적괄의온도위20℃,재각광조조건하적생장속도명현쾌우온도위15、10℃조；온도위10℃시,광조강도월고유포자체생장월쾌,온도위15、20℃시,유포자체재광조강도위20μmol/( m2·s)시적생장속도명현만우40、60μmol/( m2·s)조,이40μmol/( m2·s)조여60μmol/( m2·s)조간생장차이불명현；장자、웅배자체타쇄진행극륭묘번육,재실내배양지체장위800μm이상적유포자체,장기이지해구잠양,1개월후가생장성2 cm좌우적유체。
The effects of temperature(10, 15,and 20 ℃)and light intensity [20, 40, and 60 μmol/(m2·s)] on growth and maturation of gametophytes and juvenile sporophytes of sea weed Eisenia bicyclis introduced from Japan. The results showed that the best growth of female and male gametophytes was observed at 20 ℃, followed by at 15℃ and then at 10 ℃. The higher light intensity was, the better growth was found at 10 ℃, and there was less difference in growth among different light intensity groups as water temperature was rising. It was found that the suitable temperatures for the development and maturation of male and female gametophytes were 15 ℃ with matura-tion rate of above 95% and 20 ℃ with maturation rate of 100%,significantly higher at high light intensity than at low light intensity in 12 day culture. The juvenile sporophytes had best growth at 20 ℃ and poor growth at 15 ℃and 10 ℃ in each light intensity. The higher light intensity was, the better growth of juvenile sporophytes were at 10 ℃. There was better growth in juvenile sporophytes at 20 μmol/( m2 ·s) than at 40 μmol /( m2 ·s) and 60μmol/( m2 ·s) ,without significantly difference at 15 ℃ and 20 ℃. The cloned seedlings bred by the treated ga-metophytes were reared indoor to juvenile sporophytes with body length of 800 μm, which then were cultured to ju-veniles with body length of 2 cm in the sea in one month holding culture.