CAJ | 학술논문

目的：探讨莱芜市汉族女性5，10-亚甲基四氢叶酸还原酶（ MTHFR）、甲硫氨酸合成酶还原酶（ MTRR）基因多态性的频率特征。方法以莱芜市559例汉族女性为研究对象，检测MTHFR C677T、A1298C和MTRR A66 G的基因分型。统计分析基因多态性的频率特征，并与已报道的其他地区汉族女性的数据进行比较。结果莱芜市汉族女性的MTHFR C677T位点CC、CT、TT基因型所占比例分别为14．3％、47．6％和38．1％，该位点基因型和等位基因频率，与镇江、武汉、昆明、德阳、惠州、琼海等地差异有统计学意义（ P＜0.05）；MTHFR A1298C位点AA、AC、CC基因型所占比例分别为78.2％、19．7％和2．1％，该位点基因型和等位基因频率，与镇江、武汉、昆明、德阳、惠州、琼海等地差异有统计学意义（ P＜0．05）；MTRR A66G位点AA、AG、GG基因型所占比例分别为53．3％、38．8％和7．9％，该位点等位基因频率，与琼海汉族女性的差异有统计学意义（P＜0．05）。结论莱芜市汉族女性MTHFR、MTRR基因多态性频率分布不同于其他地区，具有地域特异性。
목적：탐토래무시한족녀성5，10-아갑기사경협산환원매（ MTHFR）、갑류안산합성매환원매（ MTRR）기인다태성적빈솔특정。방법이래무시559례한족녀성위연구대상，검측MTHFR C677T、A1298C화MTRR A66 G적기인분형。통계분석기인다태성적빈솔특정，병여이보도적기타지구한족녀성적수거진행비교。결과래무시한족녀성적MTHFR C677T위점CC、CT、TT기인형소점비례분별위14．3％、47．6％화38．1％，해위점기인형화등위기인빈솔，여진강、무한、곤명、덕양、혜주、경해등지차이유통계학의의（ P＜0.05）；MTHFR A1298C위점AA、AC、CC기인형소점비례분별위78.2％、19．7％화2．1％，해위점기인형화등위기인빈솔，여진강、무한、곤명、덕양、혜주、경해등지차이유통계학의의（ P＜0．05）；MTRR A66G위점AA、AG、GG기인형소점비례분별위53．3％、38．8％화7．9％，해위점등위기인빈솔，여경해한족녀성적차이유통계학의의（P＜0．05）。결론래무시한족녀성MTHFR、MTRR기인다태성빈솔분포불동우기타지구，구유지역특이성。
Objective To explore the 5,10-methylenetetrahydrofolate reductase( MTHFR) and 5-methyltet-rahydrofolate-homocysteine methyltransferase reductase( MTRR) gene polymorphisms among the Han women in Laiwu City.Methods A total of 559 Han women were recruited.And their oral epithelial cells were collected to extract genome DNA in order to detect gene polymorphisms of MTHFR and MTRR using fluorescence quantitative PCR.Then the results were compared with those in other cities in China.Results The frequency of MTHFR 677CC,677CT and 677TT of Han women in Laiwu city was 14.3%,46.7%and 38.1%,respectively.The frequency of MTHFR 677TT among Laiwu women was significantly different to those of Zhenjiang, Wuhan, Kunming, Deyang, Huizhou, Qionghai (P<0.05).The frequency of AA,AC,CC gene type on MTHFR A1298C was 78.2%,19.7% and 2.1%,respec-tively, the frequency was significantly different to those of Zhenjiang, Wuhan, Kunming, Deyang, Huizhou, Qionghai (P<0.05).The frequency of AA,AG,GG gene type on MTRR A66G was 53.3%,38.8%and 7.9%,respectively. The frequency of MTRR 66GG was significantly different to that of Qionghai(P<0.05).Conclusion The MTHFR, MTRR polymorphism distribution of Han women in Laiwu City has the characteristic of region specificity,respectively.