中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2014年
22期
3361-3363,3364
,共4页
李方江%杜美玲%张文婷%王晓元
李方江%杜美玲%張文婷%王曉元
리방강%두미령%장문정%왕효원
缺血预处理%超氧化物歧化酶%丙二醛%芬太尼%兔
缺血預處理%超氧化物歧化酶%丙二醛%芬太尼%兔
결혈예처리%초양화물기화매%병이철%분태니%토
Ischemic preconditioning%Superoside dismutase%Manlondialdehyde%Fentanyl%Rabbits
目的：观察芬太尼后处理联合缺血后适应对心肌缺血再灌注兔超氧化物歧化酶（ SOD）酶蛋白活力浓度、丙二醛（ MDA ）浓度的影响。方法32只日本大耳白兔采用随机区组法随机分为四组（每组8只），采用结扎兔左冠状动脉前降支30 min、复灌120 min建立心肌缺血再灌注模型。假手术组：动脉下仅穿线不结扎；缺血再灌注组：直接恢复再灌注；缺血后适应组：缺血后30 min即刻给予3轮复灌30 s/缺血30 s作为后适应，后恢复再灌注120 min；芬太尼后处理＋缺血后适应组：缺血28 min给予芬太尼5μg/kg后处理后，30 min予以缺血后适应后恢复再灌注。检测各组SOD酶蛋白活力浓度、MDA浓度。结果芬太尼后处理＋缺血后适应组较缺血后适应组外周血MDA（mmol/mL）[（3．52±0．45）比（4．42±0．47）]明显降低（F＝81.619，P＜0．01），SOD酶蛋白活力浓度（U/mL）[（178．38±20．32）比（130．18±20．44）]明显升高（F＝59.359，P＜0.01）。结论芬太尼后处理联合心肌缺血后适应显著降低心肌缺血再灌注兔外周血MDA浓度，提高SOD酶蛋白活力浓度，具有减轻心肌缺血再灌注损伤的作用。
目的：觀察芬太尼後處理聯閤缺血後適應對心肌缺血再灌註兔超氧化物歧化酶（ SOD）酶蛋白活力濃度、丙二醛（ MDA ）濃度的影響。方法32隻日本大耳白兔採用隨機區組法隨機分為四組（每組8隻），採用結扎兔左冠狀動脈前降支30 min、複灌120 min建立心肌缺血再灌註模型。假手術組：動脈下僅穿線不結扎；缺血再灌註組：直接恢複再灌註；缺血後適應組：缺血後30 min即刻給予3輪複灌30 s/缺血30 s作為後適應，後恢複再灌註120 min；芬太尼後處理＋缺血後適應組：缺血28 min給予芬太尼5μg/kg後處理後，30 min予以缺血後適應後恢複再灌註。檢測各組SOD酶蛋白活力濃度、MDA濃度。結果芬太尼後處理＋缺血後適應組較缺血後適應組外週血MDA（mmol/mL）[（3．52±0．45）比（4．42±0．47）]明顯降低（F＝81.619，P＜0．01），SOD酶蛋白活力濃度（U/mL）[（178．38±20．32）比（130．18±20．44）]明顯升高（F＝59.359，P＜0.01）。結論芬太尼後處理聯閤心肌缺血後適應顯著降低心肌缺血再灌註兔外週血MDA濃度，提高SOD酶蛋白活力濃度，具有減輕心肌缺血再灌註損傷的作用。
목적：관찰분태니후처리연합결혈후괄응대심기결혈재관주토초양화물기화매（ SOD）매단백활력농도、병이철（ MDA ）농도적영향。방법32지일본대이백토채용수궤구조법수궤분위사조（매조8지），채용결찰토좌관상동맥전강지30 min、복관120 min건립심기결혈재관주모형。가수술조：동맥하부천선불결찰；결혈재관주조：직접회복재관주；결혈후괄응조：결혈후30 min즉각급여3륜복관30 s/결혈30 s작위후괄응，후회복재관주120 min；분태니후처리＋결혈후괄응조：결혈28 min급여분태니5μg/kg후처리후，30 min여이결혈후괄응후회복재관주。검측각조SOD매단백활력농도、MDA농도。결과분태니후처리＋결혈후괄응조교결혈후괄응조외주혈MDA（mmol/mL）[（3．52±0．45）비（4．42±0．47）]명현강저（F＝81.619，P＜0．01），SOD매단백활력농도（U/mL）[（178．38±20．32）비（130．18±20．44）]명현승고（F＝59.359，P＜0.01）。결론분태니후처리연합심기결혈후괄응현저강저심기결혈재관주토외주혈MDA농도，제고SOD매단백활력농도，구유감경심기결혈재관주손상적작용。
Objective To explore the effects of fentanyl combined with ischemic post-processing on the pro-tein active concentration of superoside dismutase ( SOD) and the concentration of manlondialdehyde ( MDA) in rab-bits after myocardial ischemia and reperfusion injury.Methods The left anterior descending coronary artery were li-gated for 30 minutes and then reperfusion for 120 minutes to establish myocardial ischemia-reperfusion model of rab-bit.32 Japanese white rabbits were randomly divided into four groups by randomized block method(8 rabbits in each group):Sham-operated group(group S):seperate LAD without ligation;ischemia-reperfusion group(group I/R):re-store reperfusion directly;ischemic post conditioning group( group IPOC):restore reperfusion after ischemic post con-ditioning (filling for 30s/ischemia for 30s,three rounds);combination of fentanyl and ischemic post conditioning group( group F+IPOC):fentanyl post conditioning by ear marginal vein after ischemia 28 minutes,the dose was 5μg/kg, and after 30min,the rabbits was given ischemic post conditioning and then restored reperfusion.Then the differences in the protein active concentration of SOD and in the concentration of MDA were detected.Results The concentra-tion of MDA in the group F+IPOC were lower than that in the group IPOC[(3.52 ±0.45)mmol/mL vs (4.42 ± 0.47)mmol/mL,F =81.619,P <0.01].But the protein active concentration of SOD increased significantly [(178.38 ±20.32)U/mL vs (130.18 ±20.44)U/mL,F=59.359,P<0.01].Conclusion Fentanyl combined with ischemic post-processing can reduce the concentration of MDA and increase the protein active concentration of SOD significantly,which has the effect to decrease the myocardial ischemia and reperfusion injury.
