CAJ | 학술논문

目的：探讨建立小鼠心肌梗死模型的快速简易方法，提高动物存活率。方法：使用戊巴比妥钠麻醉小鼠，在无创性机械通气的条件下开胸，将心脏轻轻挤出胸腔后，用手术针钩断冠状动脉的左前降支（LAD），通过观察存活率、心电图改变和组织病理学染色来评价心肌梗死模型是否成功。同时与冠状动脉结扎组进行比较。结果：LAD钩断术后心电图出现ST段抬高，TTC染色可见明显的梗死区域，术后4周心肌发生纤维化；模型组的术后存活率为85%，而冠状动脉结扎组则为54%。结论：应用这种改良的新方法可以快速并成功地建立小鼠心肌梗死模型。
목적：탐토건립소서심기경사모형적쾌속간역방법，제고동물존활솔。방법：사용무파비타납마취소서，재무창성궤계통기적조건하개흉，장심장경경제출흉강후，용수술침구단관상동맥적좌전강지（LAD），통과관찰존활솔、심전도개변화조직병이학염색래평개심기경사모형시부성공。동시여관상동맥결찰조진행비교。결과：LAD구단술후심전도출현ST단태고，TTC염색가견명현적경사구역，술후4주심기발생섬유화；모형조적술후존활솔위85%，이관상동맥결찰조칙위54%。결론：응용저충개량적신방법가이쾌속병성공지건립소서심기경사모형。
Objective:To discuss the method of establishing an efficient mice myocardial infarction model with a high survival rate.Method:The mice were anesthetized with pentobarbital sodium,thoracotomy in non-invasive ventilation,gently squeeze out the heart of the chest,off the hook of the left anterior descending coronary artery with operation needle,by observing the survival rate,the change of electrocardiogram,used pathology testing to prove the model to be successful. At the same time compared with the coronary artery ligation group.Result:After off the hook of the left anterior descending coronary artery,the ST-T segment of ECG raised,the infarction area was obvious with TTC staining,myocardial fiberizes 4 weeks after operation;the survival rate of myocardial infarction model was 85%,the ligation of the coronary artery group was 54%.Conclusion:Application of this new method can establish an efficient mice myocardial infarction mode.