CAJ | 학술논문

目的：分析发热伴血小板减少综合征布尼亚病毒感染（SFTSV）感染患者的临床特点、流行病学以及SFTSV基因序列。方法：收集本市2014年4-6月某医院送检的2例重症发热伴血小板减少（SFTS）患者的血清标本，经核酸荧光PCR检测阳性确诊。对病毒核酸测序并与GenBank数据比对。结果：两名患者均有典型的SFTSV感染的临床症状，以发热、腹泻为首发症状，血常规见粒系及血小板呈进行性降低。从2例患者血清提取的核酸经测序，其基因序列与GenBank中的SFTSV进行比对，0428株（安庆）的RNA多聚酶、糖蛋白、NS蛋白的同源性与安徽代表株AHL/China/2011的同源性最高，均达到99%。0507株（滁州）上述目的基因与江苏株JS2011-013-1的同源性高达99%。结论：SFTSV在本地区散发存在，基因序列分析结果提示其来源或有差异。
목적：분석발열반혈소판감소종합정포니아병독감염（SFTSV）감염환자적림상특점、류행병학이급SFTSV기인서렬。방법：수집본시2014년4-6월모의원송검적2례중증발열반혈소판감소（SFTS）환자적혈청표본，경핵산형광PCR검측양성학진。대병독핵산측서병여GenBank수거비대。결과：량명환자균유전형적SFTSV감염적림상증상，이발열、복사위수발증상，혈상규견립계급혈소판정진행성강저。종2례환자혈청제취적핵산경측서，기기인서렬여GenBank중적SFTSV진행비대，0428주（안경）적RNA다취매、당단백、NS단백적동원성여안휘대표주AHL/China/2011적동원성최고，균체도99%。0507주（저주）상술목적기인여강소주JS2011-013-1적동원성고체99%。결론：SFTSV재본지구산발존재，기인서렬분석결과제시기래원혹유차이。
Objective:To investigate the clinical characteristics and epidemiology of patients infected with severe fever with thrombocytopenia syndrome bunyavirus(SFTSV)and the genetic background of the SFTSV. Method:Clinical and epidemiological data of two cases with severe fever with thrombocytopenia syndrome(SFTS),collected from a hospital in Nanjing during April 2014 to June 2014,were retrospectively analyzed. Nucleic acid was extracted from the serum of the two SFTSV cases. After specific polymerase chain reaction(PCR),the nucleic acid sequences were compared with those in GenBank using the BLAST facility. Result:Two patients presented typical clinical symptoms of SFTSV infection,with acute onset of fever and diarrhea. Routine blood examination showed that neutrophils and platelets progressively decreased. The results of alignment with database in GenBank,showed that RNA-dependent RNA polymerase gene,the glycoprotein gene and NS protein gene of the sample 0428(Anqing)had 99%homology with AHL/China/2011,the representative strain of AnHui Province. While the genes of sample 0507(Chuzhou)had 99% homology with JS2011-013-1,the representative strain of JiangSu Province. Conclusion:The results suggest that SFTSV occur sporadically in Nanjing and its origin might be diverse.