山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2014年
39期
15-18
,共4页
Ku70蛋白%特异性蛋白1%结肠癌%结肠肿瘤
Ku70蛋白%特異性蛋白1%結腸癌%結腸腫瘤
Ku70단백%특이성단백1%결장암%결장종류
Ku70 protein%specificity protein 1%colorectal carcinoma%colorectal neoplasms
目的：观察Ku70和特异性蛋白1（Sp1）在结肠癌组织中的表达变化，并探讨其意义。方法分别采用免疫组化SP法、RT-PCR法对结肠癌及对应癌旁正常组织中的Ku70和Sp1蛋白及mRNA进行检测，并分析二者间的相关性及与结肠癌临床病理参数的关系。结果结肠癌癌组织与癌旁正常组织中Ku70阳性表达率分别为68．9％（42/61）、36．1％（22/61），二者比较，P＜0．05；Sp1阳性表达率分别为78．7％（48/61）、26．2％（16/61），二者比较，P＜0．05。结肠癌和癌旁正常组织中Ku70 mRNA相对表达量分别为1．184±0．410、0．845±0．424，二者比较，P＜0．05；Sp1 mRNA相对表达量分别为1．280±0．311、0．912±0．362，二者比较，P＜0．05。 Ku70 mRNA和Sp1 mRNA在结肠癌组织中表达呈正相关（r＝0．398，P＝0．029）。 Ku70和Sp1蛋白表达水平与结肠癌肿瘤大小、分化程度、淋巴结转移、TNM分期有关（P均＜0．05）。结论 Ku70和Sp1在结肠癌中高表达，与结肠癌的侵袭、转移有关，二者可作为结肠癌发生、发展的预测指标。
目的：觀察Ku70和特異性蛋白1（Sp1）在結腸癌組織中的錶達變化，併探討其意義。方法分彆採用免疫組化SP法、RT-PCR法對結腸癌及對應癌徬正常組織中的Ku70和Sp1蛋白及mRNA進行檢測，併分析二者間的相關性及與結腸癌臨床病理參數的關繫。結果結腸癌癌組織與癌徬正常組織中Ku70暘性錶達率分彆為68．9％（42/61）、36．1％（22/61），二者比較，P＜0．05；Sp1暘性錶達率分彆為78．7％（48/61）、26．2％（16/61），二者比較，P＜0．05。結腸癌和癌徬正常組織中Ku70 mRNA相對錶達量分彆為1．184±0．410、0．845±0．424，二者比較，P＜0．05；Sp1 mRNA相對錶達量分彆為1．280±0．311、0．912±0．362，二者比較，P＜0．05。 Ku70 mRNA和Sp1 mRNA在結腸癌組織中錶達呈正相關（r＝0．398，P＝0．029）。 Ku70和Sp1蛋白錶達水平與結腸癌腫瘤大小、分化程度、淋巴結轉移、TNM分期有關（P均＜0．05）。結論 Ku70和Sp1在結腸癌中高錶達，與結腸癌的侵襲、轉移有關，二者可作為結腸癌髮生、髮展的預測指標。
목적：관찰Ku70화특이성단백1（Sp1）재결장암조직중적표체변화，병탐토기의의。방법분별채용면역조화SP법、RT-PCR법대결장암급대응암방정상조직중적Ku70화Sp1단백급mRNA진행검측，병분석이자간적상관성급여결장암림상병리삼수적관계。결과결장암암조직여암방정상조직중Ku70양성표체솔분별위68．9％（42/61）、36．1％（22/61），이자비교，P＜0．05；Sp1양성표체솔분별위78．7％（48/61）、26．2％（16/61），이자비교，P＜0．05。결장암화암방정상조직중Ku70 mRNA상대표체량분별위1．184±0．410、0．845±0．424，이자비교，P＜0．05；Sp1 mRNA상대표체량분별위1．280±0．311、0．912±0．362，이자비교，P＜0．05。 Ku70 mRNA화Sp1 mRNA재결장암조직중표체정정상관（r＝0．398，P＝0．029）。 Ku70화Sp1단백표체수평여결장암종류대소、분화정도、림파결전이、TNM분기유관（P균＜0．05）。결론 Ku70화Sp1재결장암중고표체，여결장암적침습、전이유관，이자가작위결장암발생、발전적예측지표。
Objective To detect the expression of Ku70 and specificity protein 1 ( Sp1 ) in colorectal cancer tissue samples.Methods Immunohistochemistry and reverse transcription-polymerase chain reaction( RT-PCR) were used to de-tect the proteins and mRNA expression of the Ku70 and Sp1 in colorectal cancer samples and matched tumor-adjacent tissue samples respectively.The correlation beween the expression of Ku70 and Sp1 and clinical pathological parameter was also analyzed.Results The positive expression rate of Ku70 in tumor tissues and matched tumor-adjacent tissues was 68.9%(42/61) and 36.1%(22/61) respectively, the difference was statistically significant(P<0.05).The positive expression rate of Sp1 was 78.6%(48/61) and 26.2%(16/61) respectively, the difference was statistically significant(P<0.05). The relative expression of Ku70 mRNA in tumor tissues and matched tumor-adjacent tissues was 1.184 ±0.410 and 0.845 ±0.424 respectively, the difference was statistically significant(P<0.05).The relative expression of Sp1 mRNA was 1.280 ±0.311 and 0.912 ±0.362, the difference was statistically significant(P<0.05).The correlation of Ku70 mRNA and Sp1 mRNA was positive(r=0.398, P=0.029).The expression levels of Ku70 and Sp1 were associated with tumor size, tumor differentiation, lymph node metastasis, TMN stage(all P<0.05).Conclusions The expressions of Ku70 and Sp1 in colorectal cancer are both higher than those in tumor-adjacent tissue, and are highly associated with the metastasis and infiltration in colorectal cancer.They may become new biomarkers in the occurrence and development of colon cancer .
