CAJ | 학술논문

目的：观察基底细胞样乳腺癌（BLBC）组织中凋亡蛋白酶活化因子1（Apaf-1）、细胞凋亡蛋白酶9（Caspase-9）的表达变化，并探讨其意义。方法采用免疫组化法检测43例BLBC（A组）、57例non-BLBC（B组）和60例癌旁正常乳腺组织（ C组） Apaf-1、Caspase-9的表达，分析两指标间及与BLBC临床病理参数的关系。结果A、B、C组Apaf-1阳性率分别为23．3％（10/43）、49．1％（28/57）、75％（45/60），两两比较，P均＜0．01；Caspase-9阳性率分别为27．9％（12/43）、54．4％（31/57）、85％（51/60），两两比较，P均＜0．01。 Apaf-1表达与BLBC肿瘤直径、淋巴结转移、临床分期有关（P均＜0．05），Caspase-9表达与BLBC淋巴结转移、临床分期有关（P均＜0．05）。BLBC组织Apaf-1与Caspase-9阳性表达呈正相关（r＝0．639，P＜0．01）。结论 BLBC组织Apaf-1、Caspase-9表达降低，两者异常表达可能与BLBC的发生、发展密切相关。
목적：관찰기저세포양유선암（BLBC）조직중조망단백매활화인자1（Apaf-1）、세포조망단백매9（Caspase-9）적표체변화，병탐토기의의。방법채용면역조화법검측43례BLBC（A조）、57례non-BLBC（B조）화60례암방정상유선조직（ C조） Apaf-1、Caspase-9적표체，분석량지표간급여BLBC림상병리삼수적관계。결과A、B、C조Apaf-1양성솔분별위23．3％（10/43）、49．1％（28/57）、75％（45/60），량량비교，P균＜0．01；Caspase-9양성솔분별위27．9％（12/43）、54．4％（31/57）、85％（51/60），량량비교，P균＜0．01。 Apaf-1표체여BLBC종류직경、림파결전이、림상분기유관（P균＜0．05），Caspase-9표체여BLBC림파결전이、림상분기유관（P균＜0．05）。BLBC조직Apaf-1여Caspase-9양성표체정정상관（r＝0．639，P＜0．01）。결론 BLBC조직Apaf-1、Caspase-9표체강저，량자이상표체가능여BLBC적발생、발전밀절상관。
Objective To observe the changes of Apaf-1, Caspase-9 expression in the basal-like breast cancer ( BLBC) tissues and explore its clinical significance.Methods The expressions of Apaf-1 and Caspase-9 were detected in 43 cases of BLBC( group A) , 57 cases of non-BLBC( group B) and 60 cases of normal breast tissue( group C) by immuno-histochemistry.The relationship between Apaf-1, Caspase-9 expression and clinicopathological features were analyzed.The correlation between Apaf-1 and Caspase-9 expression was analyzed.Results The positive expression rate of Apaf-1 in group A, B, C was 23.3%(10/43), 49.1%(28/57), 75%(45/60) respectively.The difference between each group was significant(all P<0.01).The positive expression rate of Caspase-9 in group A, B, C was 27.9%(12/43), 54.4%(31/57), 85%(51/60) respectively.The difference between each group was all significant(all P<0.01).The expres-sion of Apaf-1 was significantly correlated with the size of tumor, lymph nodes metastasis and the tumor clinical stage( all P<0.05) .The expression of Caspase-9 was significantly correlated with lymph nodes metastasis and the tumor clinical stage (all P<0.05).There correlation between the expression of Apaf-1 and Caspase-9 was significant(r=0.636, P<0.01). Conclusions Apaf-1 and Caspase-9 are lower expressed in BLBC.The abnormal expression of Apaf-1 and Caspase-9 may be closely correlated with the carcinogenesis and development of BLBC.