山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2014年
39期
5-7
,共3页
田晨%王超雨%贾勇胜%张翼鷟
田晨%王超雨%賈勇勝%張翼鷟
전신%왕초우%가용성%장익작
急性T淋巴细胞白血病%CD3+4细胞%Hes1基因%细胞周期%细胞增殖
急性T淋巴細胞白血病%CD3+4細胞%Hes1基因%細胞週期%細胞增殖
급성T림파세포백혈병%CD3+4세포%Hes1기인%세포주기%세포증식
acute T lymphoblastic leukemia%CD3+4 cell%Hes1 gene%cell cycle%proliferation
目的：观察急性T淋巴细胞白血病（ T-ALL）患者骨髓CD3＋4细胞Hes1基因表达、数量、增殖的变化，并探讨其机制。方法收集8例初治T-ALL患者及4例正常供者（健康对照）骨髓样本，real time PCR检测Hes1基因的表达，密度梯度离心法获取单个核细胞，流式细胞术检测CD3＋4细胞比例及其细胞周期，免疫磁珠法分选CD3＋4细胞，体外集落形成实验（ CFC）检测其增殖能力。构建Hes1基因过表达逆转录病毒载体，感染正常供者骨髓CD3＋4细胞后，流式细胞术分析其细胞周期的改变，CFC检测其增殖的改变。结果 T-ALL患者、健康对照CD3＋4细胞Hes1基因表达分别为3．3±0．8、1，两者比较，P＜0．05；CD3＋4细胞比例分别为0．02％±0．003％、0．06％±0．005％，两者比较，P＜0．05；CD3＋4细胞处于S期的细胞比例分别为16．2％±0．98％、28．0％±1．12％，两者比较，P＜0．05；G0期比例分别为19．0％±0．9％、9．0％±0．5％，两者比较，P＜0．05；且患者来源CD3＋4细胞的体外集落形成减少。提高正常CD3＋4细胞中Hes1基因的表达后，细胞增殖减少，进入静止期。结论 T-ALL患者CD3＋4细胞比例下降，进入静止期，体外扩增能力下降，这可能与Hes1基因的表达上调有关。
目的：觀察急性T淋巴細胞白血病（ T-ALL）患者骨髓CD3＋4細胞Hes1基因錶達、數量、增殖的變化，併探討其機製。方法收集8例初治T-ALL患者及4例正常供者（健康對照）骨髓樣本，real time PCR檢測Hes1基因的錶達，密度梯度離心法穫取單箇覈細胞，流式細胞術檢測CD3＋4細胞比例及其細胞週期，免疫磁珠法分選CD3＋4細胞，體外集落形成實驗（ CFC）檢測其增殖能力。構建Hes1基因過錶達逆轉錄病毒載體，感染正常供者骨髓CD3＋4細胞後，流式細胞術分析其細胞週期的改變，CFC檢測其增殖的改變。結果 T-ALL患者、健康對照CD3＋4細胞Hes1基因錶達分彆為3．3±0．8、1，兩者比較，P＜0．05；CD3＋4細胞比例分彆為0．02％±0．003％、0．06％±0．005％，兩者比較，P＜0．05；CD3＋4細胞處于S期的細胞比例分彆為16．2％±0．98％、28．0％±1．12％，兩者比較，P＜0．05；G0期比例分彆為19．0％±0．9％、9．0％±0．5％，兩者比較，P＜0．05；且患者來源CD3＋4細胞的體外集落形成減少。提高正常CD3＋4細胞中Hes1基因的錶達後，細胞增殖減少，進入靜止期。結論 T-ALL患者CD3＋4細胞比例下降，進入靜止期，體外擴增能力下降，這可能與Hes1基因的錶達上調有關。
목적：관찰급성T림파세포백혈병（ T-ALL）환자골수CD3＋4세포Hes1기인표체、수량、증식적변화，병탐토기궤제。방법수집8례초치T-ALL환자급4례정상공자（건강대조）골수양본，real time PCR검측Hes1기인적표체，밀도제도리심법획취단개핵세포，류식세포술검측CD3＋4세포비례급기세포주기，면역자주법분선CD3＋4세포，체외집락형성실험（ CFC）검측기증식능력。구건Hes1기인과표체역전록병독재체，감염정상공자골수CD3＋4세포후，류식세포술분석기세포주기적개변，CFC검측기증식적개변。결과 T-ALL환자、건강대조CD3＋4세포Hes1기인표체분별위3．3±0．8、1，량자비교，P＜0．05；CD3＋4세포비례분별위0．02％±0．003％、0．06％±0．005％，량자비교，P＜0．05；CD3＋4세포처우S기적세포비례분별위16．2％±0．98％、28．0％±1．12％，량자비교，P＜0．05；G0기비례분별위19．0％±0．9％、9．0％±0．5％，량자비교，P＜0．05；차환자래원CD3＋4세포적체외집락형성감소。제고정상CD3＋4세포중Hes1기인적표체후，세포증식감소，진입정지기。결론 T-ALL환자CD3＋4세포비례하강，진입정지기，체외확증능력하강，저가능여Hes1기인적표체상조유관。
Objective To study the expression of Hes1, cell cycle, cell proliferation of bone marrow CD3+4 cells in acute T lymphoblastic leukemia( T-ALL) patients.Methods Samples of 8 T-ALL patients and 4 healthy donars were collected. Hes1 expression was analyzed by real time PCR.Bone marrow mononuclear cells were isolated with Ficoll and then the pro-portion and cell cycle of CD3+4 cells were analyzed by FACS, CD3+4 cells were cultured in vitro for colony formation cells ( CFC) .After increasing the expression of Hes1 in CD3+4 cells, cell cycle was analyzed by FACS and the colony formation of CD3+4 cells were analyzed by CFC.Results Hes1 expression in T-ALL and control were 3.3 ±0.8 and 1 respectively(P<0.05).The ratio of CD3+4 cells in T-ALL and control were 0.02%±0.003%and 0.06%±0.005%respectively(P<0.05). The proportion of S phase of CD3+4 cells in T-ALL and control were 16.2%±0.98%and 28.0%±1.12%respectively(P<0.05).The proportion of G0 phase of CD3+4 cells were 19.0%±0.9%and 9.0%±0.5%respectively(P<0.05).The colo-ny formation of CD3+4 cells from patients was lower than that from normal donors.More CD3+4 cells went into quiescence and the proliferation of these cells were inhibited after overexpressing Hes1.Conclusions In T-ALL patients, the proportion of CD3+4 cells in bone marrow is lower.More of these cells go into quiescence which is related to Hes1.
