CAJ | 학술논문

采用扫描和透射电镜技术对自然成熟的条纹锯精子、卵子及精子入卵过程进行观察。观察结果显示，其精子由头部、中段和尾部三部分组成：头部主要由细胞核构成，无顶体结构；中段由线粒体、中心粒复合体(近端中心粒和基体)、袖套组成；尾部主要由轴丝组成，外部包裹质膜，轴丝为典型的“9+2”结构。卵子表面分布纵横交错的网纹，均匀分布着大小不一的微小孔，在卵壳的动物极精孔区的中央有一个受精孔。在授精后10 s即可见到精子通过受精孔进入卵子，刺激卵子发生形态变化封闭受精孔，阻止其他精子入卵，60 s可见受精孔完全封闭。
채용소묘화투사전경기술대자연성숙적조문거정자、란자급정자입란과정진행관찰。관찰결과현시，기정자유두부、중단화미부삼부분조성：두부주요유세포핵구성，무정체결구；중단유선립체、중심립복합체(근단중심립화기체)、수투조성；미부주요유축사조성，외부포과질막，축사위전형적“9+2”결구。란자표면분포종횡교착적망문，균균분포착대소불일적미소공，재란각적동물겁정공구적중앙유일개수정공。재수정후10 s즉가견도정자통과수정공진입란자，자격란자발생형태변화봉폐수정공，조지기타정자입란，60 s가견수정공완전봉폐。
To explore the fertilization mechanism of Centropristis striata and improve the fertilization rate, the ultrastructure of spermatozoon and egg and the fertilization process were observed using the scanning electron microscope (SEM) and transmission electron microscope (TEM). The results showed that the sperm of C. striata was mainly composed of the following three parts:1) head;mainly consisted with nucleus and without the acrosomal structure;2) midpiece;consisted with mitochondrion, centriolar complex (including proximal centriole and matrix) and sleeve structure; and 3) tail; consisted with axoneme that is surrounded by plasma membrane. The axoneme of C. striata was consistent with the typical“9+2”structure in most fish. The eggs were spherical, colorless, and transparent with the diameter at (0.950±0.039) mm, which belonged to pelagic eggs. A big grain of oil ball (0.182 ± 0.011mm in diameter) was observed in the egg. Overlapping curves were observed on the surface of the eggs, on which the tiny holes with different sizes (0.246±0.103 μm) were evenly distributed. A completely-opened fertilization hole was in the central part of the funneled area at the animal pole. The shell of the egg included plasma membrane, vitelline membrane and shell membrane. The shell was filled with cytoplasm. The fertilization process of C. striata was short. The sperm penetrated into the egg through the micropyle in 10 seconds after insemination. The morphological feature of the fertilized egg changed after that, and the fertilization cone and fertilization plug were observed. Finally, about 60 s after insemination, the fertilization hole closed to prevent the penetration of other sperms. This study may provide theoretical foundation to improve the fertilization rate of C. striata artificial breeding.