广西植物
廣西植物
엄서식물
GUIHAIA
2014年
6期
854-858
,共5页
甘薯茎叶%膳食纤维%正交实验%淀粉酶%脂肪酶%木瓜蛋白酶
甘藷莖葉%膳食纖維%正交實驗%澱粉酶%脂肪酶%木瓜蛋白酶
감서경협%선식섬유%정교실험%정분매%지방매%목과단백매
sweet potato stem and leaf%dietary fiber%orthogonal test%amylase%lipase%papain
通过单因素以及正交实验来探讨脂肪酶、淀粉酶和木瓜蛋白酶的加入对提取甘薯茎叶中水溶性膳食纤维的影响,并在单因素基础上对提取工艺进行正交优化,得出酶法提取的最佳工艺条件是脂肪酶添加量为0．015 g·100 mL-1,淀粉酶添加量为0．035 g·100 mL-1,木瓜蛋白酶添加量为0．075 g·100 mL-1,其中正交实验中影响因素为淀粉酶＞蛋白酶＞脂肪酶.水溶性膳食纤维的最佳提取率为1．285％.提取得到的甘薯茎叶中水溶性膳食纤维纯度高,持水力为787％,溶胀力为4．10 mL·g-1,产品具有良好的性状.
通過單因素以及正交實驗來探討脂肪酶、澱粉酶和木瓜蛋白酶的加入對提取甘藷莖葉中水溶性膳食纖維的影響,併在單因素基礎上對提取工藝進行正交優化,得齣酶法提取的最佳工藝條件是脂肪酶添加量為0．015 g·100 mL-1,澱粉酶添加量為0．035 g·100 mL-1,木瓜蛋白酶添加量為0．075 g·100 mL-1,其中正交實驗中影響因素為澱粉酶＞蛋白酶＞脂肪酶.水溶性膳食纖維的最佳提取率為1．285％.提取得到的甘藷莖葉中水溶性膳食纖維純度高,持水力為787％,溶脹力為4．10 mL·g-1,產品具有良好的性狀.
통과단인소이급정교실험래탐토지방매、정분매화목과단백매적가입대제취감서경협중수용성선식섬유적영향,병재단인소기출상대제취공예진행정교우화,득출매법제취적최가공예조건시지방매첨가량위0．015 g·100 mL-1,정분매첨가량위0．035 g·100 mL-1,목과단백매첨가량위0．075 g·100 mL-1,기중정교실험중영향인소위정분매＞단백매＞지방매.수용성선식섬유적최가제취솔위1．285％.제취득도적감서경협중수용성선식섬유순도고,지수력위787％,용창력위4．10 mL·g-1,산품구유량호적성상.
Through the single factor and orthogonal test of adding,amylase and papain effect of soluble dietary fiber on the extraction of sweet potato stem and leaf,and orthogonal optimization of extraction process on the basis of sin-gle factor were studied.The optimum condition of enzymatic extraction were as follows:adding amount of lipase was 0.015 g·100 mL-1 ,adding amount of amylase was 0.035 g·100 mL-1 ,and adding papain 0.075 g·100 mL-1 ,inclu-ding the impact of orthogonal experiment factors for amylase>protease>lipase.The best extraction rate of water-soluble dietary fiber was 1.285%.The extraction of sweet potato leaves and stems of soluble dietary fiber in high pu-rity,water holding capacity was 787%,the swelling force was 4.10 mL·g-1 ,the product had good quality.
