CAJ | 학술논문

采用扩增片段长度多态性分子标记方法,对收集于广西的11个种源33份毛竹种质进行了分析.结果表明：5对引物组合用于选择性扩增,共得到198条扩增带,其中多态性带47条(23．74％)；11个毛竹种源间的亲缘关系较近,但仍存在着一定的遗传变异,它们之间的遗传距离 D 在0．0006~0．1369之间；UPGMA 聚类分析,可将供试的11个毛竹种源分为4大类,其中昭平种源和南丹种源各为一类,与其它两类较易区分. PCA 分析结果与聚类分析结果相一致；Mantel 检验结果表明毛竹各种源遗传距离与地理距离间相关性显著(r ＝0．5558,P ＝0．0160).说明 AFLP 分子标记能将 11个种源33份毛竹种质区分开,是毛竹种质资源鉴别的有效手段和方法.
채용확증편단장도다태성분자표기방법,대수집우엄서적11개충원33빈모죽충질진행료분석.결과표명：5대인물조합용우선택성확증,공득도198조확증대,기중다태성대47조(23．74％)；11개모죽충원간적친연관계교근,단잉존재착일정적유전변이,타문지간적유전거리 D 재0．0006~0．1369지간；UPGMA 취류분석,가장공시적11개모죽충원분위4대류,기중소평충원화남단충원각위일류,여기타량류교역구분. PCA 분석결과여취류분석결과상일치；Mantel 검험결과표명모죽각충원유전거리여지리거리간상관성현저(r ＝0．5558,P ＝0．0160).설명 AFLP 분자표기능장 11개충원33빈모죽충질구분개,시모죽충질자원감별적유효수단화방법.
Thirty-three Phyllostachys edulis germplasms from 11 provenances in Guangxi were analyzed by AFLP markers.Five pairs of primer combination were used to amplify the genomic DNA.A total of 198 AFLP bands were obtained,47 (23.74%)of them were polymorphic.The genetic distance between the 11 provenance ranged from 0.000 6 to 0.136 9.The tested gemplasms can be divided into 4 categories by UPGMA clustering analysis.Nandan and Zhaoping provenances were clustered into two categories respectively.They were distinct to other two categories. PCA analysis results were consistent with the results of cluster analysis.Mantel test results showed that the correla-tions between genetic distance and geographic distance in P .edulis provenance sources were significant (r =0.555 8, P =0.555 8),and AFLP was an effective marker to identify germplasm of P .edulis .