CAJ | 학술논문

目的：设计一种新型的基于丝网印刷技术的癌胚抗原(CEA)免疫传感器，用于癌胚抗原浓度的床边检测。方法：在丝网印刷电极(SPE)上层层修饰上多壁碳纳米管(MWNTs)N，N-二甲基甲酰胺(DMF)分散液与壳聚糖纳米二氧化铈(Chitosan-Nano CeO2)溶液两种纳米材料，利用纳米铈固载癌胚抗原抗体(anti-CEA)，最后用牛血清白蛋白(BSA)封闭纳米铈上非特异位点，构建一种新型的癌胚抗原免疫传感器。采用透射电镜(TEM)及循环伏安法(CV)对修饰过程进行表征。结果：探讨不同pH、孵育温度及孵育时间对该免疫传感器性能的影响，该传感器在最适条件下对CEA响应良好，其线性范围为0～80 ng/ml，线性相关系数r=0.99825，检出限为0.08 ng/ml。结论：CEA免疫传感器具有稳定性好、灵敏度高、特异性好、结果准确可靠和可再生等优点，可应用于临床检测。
목적：설계일충신형적기우사망인쇄기술적암배항원(CEA)면역전감기，용우암배항원농도적상변검측。방법：재사망인쇄전겁(SPE)상층층수식상다벽탄납미관(MWNTs)N，N-이갑기갑선알(DMF)분산액여각취당납미이양화시(Chitosan-Nano CeO2)용액량충납미재료，이용납미시고재암배항원항체(anti-CEA)，최후용우혈청백단백(BSA)봉폐납미시상비특이위점，구건일충신형적암배항원면역전감기。채용투사전경(TEM)급순배복안법(CV)대수식과정진행표정。결과：탐토불동pH、부육온도급부육시간대해면역전감기성능적영향，해전감기재최괄조건하대CEA향응량호，기선성범위위0～80 ng/ml，선성상관계수r=0.99825，검출한위0.08 ng/ml。결론：CEA면역전감기구유은정성호、령민도고、특이성호、결과준학가고화가재생등우점，가응용우림상검측。
Objective: To design immunosensor for the determination of Carcinoembryonic Antigen(CEA) and be used in the piont-of-care test of the concentration of CEA. Methods:The immunosensor was developed by modifying multi-wall carbon nanotubes(MWNTs)which dispersed in N, N-dimethyl-formamide(DMF)on the Screen-Printed Electrodes (SPE), then Chitosan-NanoCeO2 were immobilized self-assembly. And then the antibody of CEA (anti-CEA) was absorbed on the surface of NanoCeO2 monolayer. Finally, Albumin from bovine serum (BSA) blocked the non-specific adsorption site.The modification process was characterized by transmisCeOn electronmicroscope(TEM)and cyclic voltammetry(CV). The factors possibly influenced the performance of the proposed immunosensor were studied in detail. Results:Under optimal conditions,the obtained immunosensor exhibited good electrochemical behavior to CEA in a linear range of 0 to 80 ng/ml (r=0.99825), with a detection limit of 0.08 ng/ml. Conclusion: The immunosensor prepared using this method has advantages of good stability, regeneration, high sensitivity and specificity. Thus, the method can be used for clinical application.