CAJ | 학술논문

菲利普孢囊线虫(Heterodera filipjevi)是我国黄淮麦区新发现的一种病原线虫,但在小麦属中缺乏有效抗源。为从小麦近缘种属中发掘抗病资源,采用室内接种平均单株雌虫鉴定法和相对抗病指数法,从34份卵穗山羊草(Aegilops geniculata Roth)材料中筛选出6份抗H. filipjevi的种质材料,其中PI542187表现高抗, PI564186、PI573396、PI374365、PI361880和 PI374365表现抗病。对中国春–卵穗山羊草染色体附加系抗性鉴定,发现卵穗山羊草7Ug和5Mg附加系的单株白雌虫数明显低于中国春。连续两年又对17份小麦–卵穗山羊草5Mg-5D 易位系材料进行抗性鉴定现发,5Mg464、5Mg466和5Mg457抗性较好。
비리보포낭선충(Heterodera filipjevi)시아국황회맥구신발현적일충병원선충,단재소맥속중결핍유효항원。위종소맥근연충속중발굴항병자원,채용실내접충평균단주자충감정법화상대항병지수법,종34빈란수산양초(Aegilops geniculata Roth)재료중사선출6빈항H. filipjevi적충질재료,기중PI542187표현고항, PI564186、PI573396、PI374365、PI361880화 PI374365표현항병。대중국춘–란수산양초염색체부가계항성감정,발현란수산양초7Ug화5Mg부가계적단주백자충수명현저우중국춘。련속량년우대17빈소맥–란수산양초5Mg-5D 역위계재료진행항성감정현발,5Mg464、5Mg466화5Mg457항성교호。
Heterodera filipjevi is a pathogenic nematode of wheat newly discovered in Huang-Huai Plain of China. However, germplasm resistant to H. filipjevi has been rarely found in genus Triticum. To mine resistance genes from wheat relative species, we identified the resistance to H. filipjevi in 34 accessions of Aegilops geniculata by inoculating H. filipjevi Xuchang population with the mean female number per plant method and relative resistance index methods in greenhouse. Six candidates of germplasm were identified including PI542187 in high resistance and other five accessions (PI564186, PI573396, PI374365, PI361880, and PI374365) in resistance. A set of Chinese Spring (CS)–Ae. geniculata addition lines were used to chromosomally locate the resis-tance gene(s). Lines 7Ug and 5Mg showed obviously fewer females per plant than CS. In two consecutive years, 17 CS–Ae. geni-culata 5Mg-5D translocation lines were further tested for resistance against H. filipjevi. Lines 5Mg464, 5Mg466, and 5Mg457 exhibited resistance to H. filipjevi.