作物学报
作物學報
작물학보
ACTA AGRONOMICA SINICA
2014年
11期
1905-1913
,共9页
陈坤梅%李宏伟%林凡云%陈耀锋%李滨%郑琪%李振声
陳坤梅%李宏偉%林凡雲%陳耀鋒%李濱%鄭琪%李振聲
진곤매%리굉위%림범운%진요봉%리빈%정기%리진성
小麦%大麦条斑病毒(BSMV)%病毒介导的基因沉默%基因功能%光氧化
小麥%大麥條斑病毒(BSMV)%病毒介導的基因沉默%基因功能%光氧化
소맥%대맥조반병독(BSMV)%병독개도적기인침묵%기인공능%광양화
Triticum aestivum%Barley stripe mosaic virus%VIGS%Genomic function%Photo-oxidative stress
为了鉴定可能的小麦抗光氧化基因,利用大麦条斑病毒(barley stripe mosaic virus, BSMV)介导的基因沉默(virus-induced gene silencing, VIGS)系统,对6个小偃54响应强光的基因进行了沉默表达研究。以BSMV:GFP植株为对照,分析了这些基因的减量表达植株在低温强光、DCMU、MV、H2O2等处理下的 PSII 最大光化学效率(Fv/Fm)和光合性能指数(P.I.)、MDA含量及整株生物量变化。结果显示, Ta23008和Ta92165均参与小麦对低温强光、DCMU、MV和H2O2等胁迫的响应过程; Ta106078参与小麦对DCMU、MV和H2O2等胁迫的响应过程; Ta27787参与小麦对低温强光、DCMU 和 H2O2等胁迫的响应过程; Ta24695参与小麦对低温强光和 H2O2胁迫的响应过程;而 Ta119251仅参与小麦对DCMU的响应过程。此外, Ta23008、Ta92165、Ta106078、Ta119251四个基因被抑制表达后,其转化株系的生物量比对照显著降低,推测其可能参与调控小麦生物量的积累。
為瞭鑒定可能的小麥抗光氧化基因,利用大麥條斑病毒(barley stripe mosaic virus, BSMV)介導的基因沉默(virus-induced gene silencing, VIGS)繫統,對6箇小偃54響應彊光的基因進行瞭沉默錶達研究。以BSMV:GFP植株為對照,分析瞭這些基因的減量錶達植株在低溫彊光、DCMU、MV、H2O2等處理下的 PSII 最大光化學效率(Fv/Fm)和光閤性能指數(P.I.)、MDA含量及整株生物量變化。結果顯示, Ta23008和Ta92165均參與小麥對低溫彊光、DCMU、MV和H2O2等脅迫的響應過程; Ta106078參與小麥對DCMU、MV和H2O2等脅迫的響應過程; Ta27787參與小麥對低溫彊光、DCMU 和 H2O2等脅迫的響應過程; Ta24695參與小麥對低溫彊光和 H2O2脅迫的響應過程;而 Ta119251僅參與小麥對DCMU的響應過程。此外, Ta23008、Ta92165、Ta106078、Ta119251四箇基因被抑製錶達後,其轉化株繫的生物量比對照顯著降低,推測其可能參與調控小麥生物量的積纍。
위료감정가능적소맥항광양화기인,이용대맥조반병독(barley stripe mosaic virus, BSMV)개도적기인침묵(virus-induced gene silencing, VIGS)계통,대6개소언54향응강광적기인진행료침묵표체연구。이BSMV:GFP식주위대조,분석료저사기인적감량표체식주재저온강광、DCMU、MV、H2O2등처리하적 PSII 최대광화학효솔(Fv/Fm)화광합성능지수(P.I.)、MDA함량급정주생물량변화。결과현시, Ta23008화Ta92165균삼여소맥대저온강광、DCMU、MV화H2O2등협박적향응과정; Ta106078삼여소맥대DCMU、MV화H2O2등협박적향응과정; Ta27787삼여소맥대저온강광、DCMU 화 H2O2등협박적향응과정; Ta24695삼여소맥대저온강광화 H2O2협박적향응과정;이 Ta119251부삼여소맥대DCMU적향응과정。차외, Ta23008、Ta92165、Ta106078、Ta119251사개기인피억제표체후,기전화주계적생물량비대조현저강저,추측기가능삼여조공소맥생물량적적루。
Functional analysis of photo-oxidative stress responsive genes in wheat (Triticum aestivum L.) may benefit wheat im-provement for high radiation use efficiency. A Chinese variety Xiaoyan 54 developed from distant hybridization between common wheat (T. aestivum, 2n=42) and tall wheatgrass (Thinopyrum ponticum, 2n=70) shows significant tolerance to high light induced photo-oxidative stress. Based on previous transcriptome analysis of Xiaoyan 54 in response to high light stress, six genes were selected in this study to assess their possible roles in photo-oxidative stress response using barley stripe mosaic virus (BSMV) mediated virus-induced gene silencing (VIGS) system in Xiaoyan 54. The BSMV induced silencing of the targeted genes together with the BSMV:GFP control plants were exposed to low temperature and high light (LTHL), N-(3,4-dichlorophenyl)-N’,N’-dimethylurea (DCMU), methylviologen (MV), and hydrogen peroxide (H2O2) stress, respectively. The maximum photochemical efficiency of photosystem II (Fv/Fm), the photosynthetic performance index (P.I.), malondialdehyde (MDA) content, and biomass were evaluated. The results showed that Ta23008 and Ta92165 were involved in the responses of wheat to LTHL, DCMU, MV, and H2O2, respectively. Ta106078 was responsible for wheat tolerance to DCMU, MV, and H2O2 while Ta27787 was responsible for LTHL, DCMU, and H2O2 stress. Ta24695 participated in the response of wheat to both LTHL and H2O2. However, Ta119251 seemed to be only responsible for the DCMU stress in wheat. Additionally, four genes, Ta23008, Ta92165, Ta106078, and Ta119251, were likely to regulate biomass accumulation because the biomass was significantly reduced when they were silenced in wheat. These results provided new hints toward understanding the molecular mechanism of tolerance to photo-oxidative stress in Xiaoyan 54.