CAJ | 학술논문

建立了兽药中非法添加药物阿托品的超高效液相色谱－串联质谱（ UPLC－MS／MS）的检测方法。样品经提取稀释，采用 BEH C18柱为分离柱，外标法定量。结果表明，阿托品在0．5～10 ng／mL范围内线性关系良好，相关系数大于0．99。在4～6 mg／g（或mg／mL）添加浓度范围内阿托品的平均回收率97．4％～110．9％，批内变异系数均小于10％，检测限为0．2 mg／g（或mg／mL），定量限为0．5 mg／g（或mg／mL）。该方法简便、灵敏度高、重现性好，适用于兽药中非法添加阿托品的含量测定。
건립료수약중비법첨가약물아탁품적초고효액상색보－천련질보（ UPLC－MS／MS）적검측방법。양품경제취희석，채용 BEH C18주위분리주，외표법정량。결과표명，아탁품재0．5～10 ng／mL범위내선성관계량호，상관계수대우0．99。재4～6 mg／g（혹mg／mL）첨가농도범위내아탁품적평균회수솔97．4％～110．9％，비내변이계수균소우10％，검측한위0．2 mg／g（혹mg／mL），정량한위0．5 mg／g（혹mg／mL）。해방법간편、령민도고、중현성호，괄용우수약중비법첨가아탁품적함량측정。
A method based on ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) was developed for determination of atropine in veterinary drugs. The analyte was extracted and diluted with water, with BEH C18 as the analytical column. Quantification of the analyte was using external standard method. The results indicated that the correlations of atropine were greater than 0. 99 in the range of 0.5~10 ng/mL. The average recoveries of the drug ranged from 97.4%~110.9% at spiked levels of 4~6 mg/g (mg/mL) with RSD less than 10%. The detection limit and quantification limit of the method were 0.2 mg/g(mg/mL) and 0.5 mg/g(mg/mL). The method was convenient,rapid,sensitive and reproducible for monitoring atropine illegally added in veterinary drugs.