CAJ | 학술논문

根据猪伪狂犬病毒（ PRV） gE基因序列保守区段，设计一对特异性引物，通过优化反应条件，建立了可区分猪伪狂犬病毒野毒株与基因缺失疫苗株的PCR检测方法，并对该方法的敏感性、特异性和重复性进行了验证。结果显示，该PCR方法可扩增出388 bp的目的片段；对模板的最低检测量为1．1 pg；与猪圆环病毒Ⅱ型、猪细小病毒、猪支原体、猪瘟病毒、猪繁殖与呼吸综合征病毒、猪乙型脑炎病毒、猪流行性腹泻病毒无交叉反应，具有高特异性。采用建立的PCR方法对2014年以来全国不同地区81个猪场421份疑似病料进行检测，发现PRV猪场平均阳性率为35．80％，样品平均阳性率为25．42％。该方法灵敏度高、特异性强、重复性好，可用于PRV的临床诊断和流行病学调查。
근거저위광견병독（ PRV） gE기인서렬보수구단，설계일대특이성인물，통과우화반응조건，건립료가구분저위광견병독야독주여기인결실역묘주적PCR검측방법，병대해방법적민감성、특이성화중복성진행료험증。결과현시，해PCR방법가확증출388 bp적목적편단；대모판적최저검측량위1．1 pg；여저원배병독Ⅱ형、저세소병독、저지원체、저온병독、저번식여호흡종합정병독、저을형뇌염병독、저류행성복사병독무교차반응，구유고특이성。채용건립적PCR방법대2014년이래전국불동지구81개저장421빈의사병료진행검측，발현PRV저장평균양성솔위35．80％，양품평균양성솔위25．42％。해방법령민도고、특이성강、중복성호，가용우PRV적림상진단화류행병학조사。
Based on the conserved region of gE gene, a pair of primers were designed, and a PCR assay which can distinguish wild strain and vaccine strains with gene deletion was established by improving condition. Meanwhile, the sensibility, specificity and repeatability of the assays were verified. The results demonstrated that the established PCR assay could amplify 388 bp target fragment, and could detect 1.1 pg DNA and no cross with porcine circovirus type 2, porcine parvovirus, Mycoplasma hyopneumoniae, classical swine fever virus, porcine reproductive and respiratory syndrome virus, swine Japanese encephalitis virus, porcine epidemic diarrhea virus. Using the established PCR method, 421 suspected materials from 81 pig farms since 2014 were detected, the positive detection rate of pig farms and the materials were 35.80% and 25.42%, respectively. The PCR assay was sensitive, specific and accurate that could be used for massive samples detection and diagnosis of PRV infection.