CAJ | 학술논문

目的：探讨CPPs-BDI-1-载蟾酥白蛋白纳米微球三联体杀伤 EJ人膀胱癌细胞的实验研究。方法采用异型双功能连接剂SPDP耦联穿膜肽增强型绿色荧光蛋白( TAT-EGFP)、抗人膀胱癌单克隆抗体(BDI-1)、载蟾酥白蛋白纳米微球(Bufalin-NS),构建载蟾酥白蛋白纳米微球三联体(CPPs-BDI-1-Bufa-lin-NS)。取CPPs-BDI-1-Bufalin-NS三联体实验组和蟾酥对照组,终浓度分别为1×10-8mol/L、1×10-6mol/L,通过MTT法检测培养12小时、24小时、36小时、48小时的EJ人膀胱癌细胞存活率,检测CPPs-BDI-1-Bufalin-NS三联体对EJ人膀胱癌细胞的抑制作用。结果 CPPs-BDI-1-Bufalin-NS三联体实验组杀伤人膀胱癌细胞作用明显强于蟾酥对照组。结论 CPPs-BDI-1-Bufalin-NS三联对人膀胱癌细胞有明显的杀伤效果,为后续动物实验奠定基础。
목적：탐토CPPs-BDI-1-재섬소백단백납미미구삼련체살상 EJ인방광암세포적실험연구。방법채용이형쌍공능련접제SPDP우련천막태증강형록색형광단백( TAT-EGFP)、항인방광암단극륭항체(BDI-1)、재섬소백단백납미미구(Bufalin-NS),구건재섬소백단백납미미구삼련체(CPPs-BDI-1-Bufa-lin-NS)。취CPPs-BDI-1-Bufalin-NS삼련체실험조화섬소대조조,종농도분별위1×10-8mol/L、1×10-6mol/L,통과MTT법검측배양12소시、24소시、36소시、48소시적EJ인방광암세포존활솔,검측CPPs-BDI-1-Bufalin-NS삼련체대EJ인방광암세포적억제작용。결과 CPPs-BDI-1-Bufalin-NS삼련체실험조살상인방광암세포작용명현강우섬소대조조。결론 CPPs-BDI-1-Bufalin-NS삼련대인방광암세포유명현적살상효과,위후속동물실험전정기출。
Objective To explore the experimental research of CPPs–BDI-1–bufalin–Loaded albumin nanopar-ticle triad killing EJ human bladder cancer cells. Method Use SPDP decoupling league to build nanometer micro-spheres triplet CPPs-BDI-Bufalin-NS with the preparation of TAT-EGFP, BDI-1-NS, Bufalin-NS. Take CPPs-BDI-1-Bufalin-NS triplets as experimental group and bufalin control group, with the final concentration of 1×10-8mol/L and 1×10-6mol/L, by MTT method detecting the survival rate of EJ human bladder cancer cells after 12h, 24h, 36h, 48h, which detect the inhibition effect of CPPs-BDI-1-Bufalin-NS triplets killing human bladder cancer cells. Result The inhibition effect CPPs-BDI-1-Bufalin-NS triplet killing EJ human bladder cancer cells was obviously stronger than the bufalin control group. Conclusion It was a obvious killing effect that CPPs-BDI-1-Bufalin-NS trip-lets killed human bladder cancer cells and laied the foundation for subsequent animal experiment.