CAJ | 학술논문

目的：研究人体片形吸虫病ELISA检测试剂盒的检测效果。方法：采用云南大理本地采集的牛体片形吸虫成虫制备可溶性粗抗原，包被反应板，采用人体片形吸虫病ELISA法（FAWA-ELISA）进行研究，分别检测26份片形吸虫患者血清、102份其他寄生虫病患者血清和25份健康人血清，评价检测试剂盒的检测效果。结果：检测试剂盒的敏感性为100.0%、特异性分别为95.28%（95%CI：98.9%～91.2%），约登指数分别为0.95。结论：FAWA-ELISA试剂盒检测效果较好，且成本相对较低，可适宜用于云南片形吸虫病流行区流行病学筛查，减轻病原学检测的工作量。
목적：연구인체편형흡충병ELISA검측시제합적검측효과。방법：채용운남대리본지채집적우체편형흡충성충제비가용성조항원，포피반응판，채용인체편형흡충병ELISA법（FAWA-ELISA）진행연구，분별검측26빈편형흡충환자혈청、102빈기타기생충병환자혈청화25빈건강인혈청，평개검측시제합적검측효과。결과：검측시제합적민감성위100.0%、특이성분별위95.28%（95%CI：98.9%～91.2%），약등지수분별위0.95。결론：FAWA-ELISA시제합검측효과교호，차성본상대교저，가괄의용우운남편형흡충병류행구류행병학사사，감경병원학검측적공작량。
Objective: To evaluate the detection effect of the ELISA kit for the human fasciolasis. Methods: The soluble crude antigen from the cattle Fasciola spp. adults were collected in Dali. Then, the ELISA was done. The detection results of serum were from the 26 fasciolasis patients, and 102 other parasite infected patients. 25 healthy people were evaluated. Results:The sensitivity of the the ELISA kit was 100.0%;the specificity was 95.28%(95%CI:98.9%-91.2%), and the Youden index was 0.95. Conclusion:For the FAWA-ELISA kit, it not only can get the reliable detection results, but also has lower cost. Furthermore, it is suitable for the epidemiology investigation in Yunnan fasciolasis epidemic area, and can reduce the workload of pathogen detection.