大理学院学报
大理學院學報
대이학원학보
JOURNAL OF DALI COLLEGE
2014年
10期
10-13
,共4页
李代航%朱万芸%段素英%赖泳
李代航%硃萬蕓%段素英%賴泳
리대항%주만예%단소영%뢰영
微毛诃子%半数致死量%肠肌
微毛訶子%半數緻死量%腸肌
미모가자%반수치사량%장기
terminalia chebula Retz%lethal dose%myenteric
目的：研究微毛诃子树皮醇提取液对小鼠的半数致死量（LD50）以及在体肠肌的影响，并且观察其对豚鼠离体肠肌的影响。方法：按Bliss法设计实验，对小鼠灌胃给药，观察5 d内小鼠的毒性反应和死亡情况，以小鼠急性死亡率为指标，测定微毛诃子提取液对小鼠的LD50；采用炭末推进实验观察药物对在体肠肌的影响；以豚鼠离体肠肌收缩幅度、张力为指标，采用离体标本运动实验法观察微毛诃子树皮醇提液对豚鼠离体肠肌的影响及其作用机制。结果：微毛诃子树皮提取液小鼠灌胃给药的LD50为5.69 g/kg，LD50的95%的可信限为4.60~7.05 g/kg；微毛诃子树皮的醇提液对小鼠在体肠肌和豚鼠离体肠肌都具有明显的舒张作用，且组胺对微毛诃子树皮的醇提液的抑制作用具有拮抗作用。结论：微毛诃子树皮的醇提取液具有一定的毒性，同时微毛诃子树皮醇提液能在一定程度上抑制肠肌的收缩，且作用机制可能与阻断组织胺受体有关。
目的：研究微毛訶子樹皮醇提取液對小鼠的半數緻死量（LD50）以及在體腸肌的影響，併且觀察其對豚鼠離體腸肌的影響。方法：按Bliss法設計實驗，對小鼠灌胃給藥，觀察5 d內小鼠的毒性反應和死亡情況，以小鼠急性死亡率為指標，測定微毛訶子提取液對小鼠的LD50；採用炭末推進實驗觀察藥物對在體腸肌的影響；以豚鼠離體腸肌收縮幅度、張力為指標，採用離體標本運動實驗法觀察微毛訶子樹皮醇提液對豚鼠離體腸肌的影響及其作用機製。結果：微毛訶子樹皮提取液小鼠灌胃給藥的LD50為5.69 g/kg，LD50的95%的可信限為4.60~7.05 g/kg；微毛訶子樹皮的醇提液對小鼠在體腸肌和豚鼠離體腸肌都具有明顯的舒張作用，且組胺對微毛訶子樹皮的醇提液的抑製作用具有拮抗作用。結論：微毛訶子樹皮的醇提取液具有一定的毒性，同時微毛訶子樹皮醇提液能在一定程度上抑製腸肌的收縮，且作用機製可能與阻斷組織胺受體有關。
목적：연구미모가자수피순제취액대소서적반수치사량（LD50）이급재체장기적영향，병차관찰기대돈서리체장기적영향。방법：안Bliss법설계실험，대소서관위급약，관찰5 d내소서적독성반응화사망정황，이소서급성사망솔위지표，측정미모가자제취액대소서적LD50；채용탄말추진실험관찰약물대재체장기적영향；이돈서리체장기수축폭도、장력위지표，채용리체표본운동실험법관찰미모가자수피순제액대돈서리체장기적영향급기작용궤제。결과：미모가자수피제취액소서관위급약적LD50위5.69 g/kg，LD50적95%적가신한위4.60~7.05 g/kg；미모가자수피적순제액대소서재체장기화돈서리체장기도구유명현적서장작용，차조알대미모가자수피적순제액적억제작용구유길항작용。결론：미모가자수피적순제취액구유일정적독성，동시미모가자수피순제액능재일정정도상억제장기적수축，차작용궤제가능여조단조직알수체유관。
Objective:To research the micro-ethanol extract of terminalia bark median lethal dose in mice (LD50) and the influence of intestinal muscle in vivo, and observe the effect on the intestinal muscle of Guinea pig in vitro. Methods: The experiments were designed according to Bliss. Intragastric administration and observation on the toxicity and deaths of 5 d were done in mice. With acute mortality in mice as indicators, determination of extract of terminalia LD50 for mice was done; charcoal powder and advancing experimental studied the effects of drugs on the intestinal muscle; based on isolated Guinea pig intestinal smooth contraction and tension indicator, isolated specimen movement experiment was observed by micro-terminalia bark alcohol extract on isolated intestinal muscle of Guinea pigs and its mechanism of action. Results: The LD50 of terminalia bark extract in mice was 5.69 g/kg. Its 95%confidence limit was 4.60-7.05 g/kg. Minimally the ethanol extract of terminalia bark on mice intestinal muscle in vivo and in vitro in Guinea pigs has marked the vasodilator effect of intestinal muscle and histamine inhibitory effect of ethanol extract of minimal terminalia antagonism. Conclusion:Alcohol extract of terminalia bark has some toxicity, while micro-terminalia bark alcohol extract to a certain extent inhibits intestinal muscle contractions, and mechanism of action of histamine receptor-related.
