CAJ | 학술논문

目的：研究美洲大蠊提取物CII-3抗HSV-2作用。方法：通过MTT法和CPE法测定美洲大蠊提取物CII-3抗HSV-2活性，计算其TC50、IC50及TI。以雌性小鼠生殖器疱疹为动物模型，在小鼠阴道内注射50μL HSV-2病毒原液，1次/d，连续7 d造模。以ACV为阳性对照，采用灌胃给药治疗10 d。应用ELISA试剂盒检测小鼠血清中TNF-α、IFN-γ、IL-2的水平变化；称量小鼠免疫器官重量，计算脏器指数。结果：美洲大蠊提取物CII-3有抑制HSV-2致细胞病变的作用，其TC50值为190.55μg/mL，IC50值为28.71μg/mL，TI值为6.64μg/mL；美洲大蠊提取物CII-3能够提高小鼠血清中TNF-α、IFN-γ、IL-2的水平，同时能够使小鼠的免疫器官重量增加。结论：美洲大蠊提取物CII-3有抗HSV-2作用，其抗病毒作用可能是通过提高机体免疫功能来实现。
목적：연구미주대렴제취물CII-3항HSV-2작용。방법：통과MTT법화CPE법측정미주대렴제취물CII-3항HSV-2활성，계산기TC50、IC50급TI。이자성소서생식기포진위동물모형，재소서음도내주사50μL HSV-2병독원액，1차/d，련속7 d조모。이ACV위양성대조，채용관위급약치료10 d。응용ELISA시제합검측소서혈청중TNF-α、IFN-γ、IL-2적수평변화；칭량소서면역기관중량，계산장기지수。결과：미주대렴제취물CII-3유억제HSV-2치세포병변적작용，기TC50치위190.55μg/mL，IC50치위28.71μg/mL，TI치위6.64μg/mL；미주대렴제취물CII-3능구제고소서혈청중TNF-α、IFN-γ、IL-2적수평，동시능구사소서적면역기관중량증가。결론：미주대렴제취물CII-3유항HSV-2작용，기항병독작용가능시통과제고궤체면역공능래실현。
Objective: To study the anti-HSV-2 effect of CII-3 extracted from Periplaneta Americana. Methods: Anti-HSV-2 activity of CII-3 extracted from Periplaneta Americana was observed and determined with MTT assay and cytopathic effect (CPE) assay and the value of TC50, IC50 and TI in the extract was calculated. Female mice with genital herpes were used as animal models. 50μL of HSV-2 was injected into mice vaginas once a day with 7 consecutive days. With acyclovir as the positive control, intragastric administration was adopted as treatment for 10 days. The ELISA KIT was used to test the concentration of vascular tumor necrosis factor-α (TNF-α), Interferon-γ (IFN-γ) and Interleukin-2 (IL-2); the immune organs were weighed to calculate the organ index. Results: CII-3 extracted from Periplaneta Americana had anti-HSV-2 activity. The TC50 value was 190.55 μg/mL, IC50 value was 28.71μg/mL and TI value was 6.64μg/mL. CII-3 had the function of increasing TNF-α, IFN-γ, IL-2 and could increase the weight of the immune organs in mice. Conclusion:CII-3 extracted from Periplaneta Americana had the effect of anti-HSV-2 by boosting the body's immunity.