陕西医学杂志
陝西醫學雜誌
협서의학잡지
SHAANXI MEDICAL JOURNAL
2014年
11期
1449-1452
,共4页
周雷鸣%张玲%房明岗%贾济
週雷鳴%張玲%房明崗%賈濟
주뢰명%장령%방명강%가제
白藜芦醇%小神经胶质细胞/药物作用%激活%脂多糖类/生理学%沉默信息调节蛋白%炎症
白藜蘆醇%小神經膠質細胞/藥物作用%激活%脂多糖類/生理學%沉默信息調節蛋白%炎癥
백려호순%소신경효질세포/약물작용%격활%지다당류/생이학%침묵신식조절단백%염증
Resveratrol%Microglia/drug effects%Activation%Lipopolysaccharides/physiology%Silent information regulator proteins%Inflammation
目的：探讨白藜芦醇（Resv）抑制脂多糖（LPS）引起的小胶质细胞激活的作用及机制。方法：将小胶质细胞分为对照组、100ng／ml的LPS刺激组、25μM的Resv＋LPS组、沉默信息调节因子1（SIRT1）‐siRNA＋Resv＋LPS组和SC‐siRNA＋Resv＋LPS组，24h后，采用酶联免疫法（ELISA）检测细胞培养上清液中促炎症因子白细胞介素1β（IL‐1β）、IL‐6和肿瘤坏死因子‐α（TNF‐α）的浓度，Westernblot检测诱导型一氧化氮合酶（iNOS）表达，相差显微镜观测细胞形态。结果：LPS暴露可显著激活小胶质细胞，增加促炎症因子IL‐1β、IL‐6和TNF‐α的分泌和iNOS表达，细胞形态从静息状态的多角形变为激活状态的圆形，白藜芦醇可显著抑制LPS对小胶质细胞产生的上述激活作用，而SIRT1‐siRNA显著逆转了白藜芦醇产生的抑制小胶质细胞激活的作用。结论：白藜芦醇可通过SIRT1减轻LPS引起小胶质细胞激活。
目的：探討白藜蘆醇（Resv）抑製脂多糖（LPS）引起的小膠質細胞激活的作用及機製。方法：將小膠質細胞分為對照組、100ng／ml的LPS刺激組、25μM的Resv＋LPS組、沉默信息調節因子1（SIRT1）‐siRNA＋Resv＋LPS組和SC‐siRNA＋Resv＋LPS組，24h後，採用酶聯免疫法（ELISA）檢測細胞培養上清液中促炎癥因子白細胞介素1β（IL‐1β）、IL‐6和腫瘤壞死因子‐α（TNF‐α）的濃度，Westernblot檢測誘導型一氧化氮閤酶（iNOS）錶達，相差顯微鏡觀測細胞形態。結果：LPS暴露可顯著激活小膠質細胞，增加促炎癥因子IL‐1β、IL‐6和TNF‐α的分泌和iNOS錶達，細胞形態從靜息狀態的多角形變為激活狀態的圓形，白藜蘆醇可顯著抑製LPS對小膠質細胞產生的上述激活作用，而SIRT1‐siRNA顯著逆轉瞭白藜蘆醇產生的抑製小膠質細胞激活的作用。結論：白藜蘆醇可通過SIRT1減輕LPS引起小膠質細胞激活。
목적：탐토백려호순（Resv）억제지다당（LPS）인기적소효질세포격활적작용급궤제。방법：장소효질세포분위대조조、100ng／ml적LPS자격조、25μM적Resv＋LPS조、침묵신식조절인자1（SIRT1）‐siRNA＋Resv＋LPS조화SC‐siRNA＋Resv＋LPS조，24h후，채용매련면역법（ELISA）검측세포배양상청액중촉염증인자백세포개소1β（IL‐1β）、IL‐6화종류배사인자‐α（TNF‐α）적농도，Westernblot검측유도형일양화담합매（iNOS）표체，상차현미경관측세포형태。결과：LPS폭로가현저격활소효질세포，증가촉염증인자IL‐1β、IL‐6화TNF‐α적분비화iNOS표체，세포형태종정식상태적다각형변위격활상태적원형，백려호순가현저억제LPS대소효질세포산생적상술격활작용，이SIRT1‐siRNA현저역전료백려호순산생적억제소효질세포격활적작용。결론：백려호순가통과SIRT1감경LPS인기소효질세포격활。
Objective:To investigate the role of resveratrol (Resv) in lipopolysaccharide (LPS)‐induced microglial activation .Methods :Microglial cells were divided into control ;LPS exposure group :cells were exposed to 100 ng/ml LPS for 24 h;Resv+LPS group :cells were exposed 25μM Resv plus 100 ng/ml LPS for 24 h;silent information regulator 1 (SIRT1)‐siRNA+Resv+LPS group:cells were incubated in the medium containing SIRT 1‐siRNA for 6 h ,and then exposed to 25μM Resv plus 100 ng/ml LPS for 24 h ;SC‐siRNA+Resv+LPS group:cells were incubated in the medium containing scramble siRNA for 6 h ,and then exposed to 25 μM Resv plus 100 ng/ml LPS for 24 h .Interleukin‐1β(IL‐1β) ,IL‐6 and tumor necrosis factor‐α(TNF‐α) in the supernatant was evaluated by enzyme‐linked immunosorbent assay (ELISA) ,the expression of inducible nitric oxide synthase (iNOS) was as‐sessed by western blot and cellular morphology was observed by a phase contrast microscope .Results :Microglial cells were activated by LPS ,increasing the release of IL‐1β,IL‐6 and TNF‐αcompared with the control .In addition , LPS enlarged the cell .Resveratrol inhibited the LPS‐induced effect on microglial cells ,and SIRT1‐siRNA partially reversed resveratrol‐induced anti‐inflammation .Conclusion :Resveratrol attenuates LPS‐induced microglial activation via SIRT1 .
