CAJ | 학술논문

为了解硫丹对棕壤生态系统的毒性影响，采用室内避光培养模拟实验，设定硫丹在土壤中的浓度分别为0.1、1、10 mg·kg-1，取样时间为7、14、21、28 d，测定土壤中脲酶、脱氢酶和β-葡糖苷酶活性的变化，同时采用变性梯度凝胶电泳（PCR-DGGE）分子指纹技术研究硫丹在不同培养时间对土壤中细菌群落结构的影响。结果表明：土壤脲酶活性与对照相比无显著差异；脱氢酶活性受到显著抑制，且随着硫丹浓度增大，抑制作用增强；土壤β-葡糖苷酶活性则被显著激活。经BLAST程序与GenBank数据库进行比对分析，低浓度硫丹处理在培养周期28 d内，对棕壤中细菌群落结构没有产生显著的干扰作用。
위료해류단대종양생태계통적독성영향，채용실내피광배양모의실험，설정류단재토양중적농도분별위0.1、1、10 mg·kg-1，취양시간위7、14、21、28 d，측정토양중뇨매、탈경매화β-포당감매활성적변화，동시채용변성제도응효전영（PCR-DGGE）분자지문기술연구류단재불동배양시간대토양중세균군락결구적영향。결과표명：토양뇨매활성여대조상비무현저차이；탈경매활성수도현저억제，차수착류단농도증대，억제작용증강；토양β-포당감매활성칙피현저격활。경BLAST정서여GenBank수거고진행비대분석，저농도류단처리재배양주기28 d내，대종양중세균군락결구몰유산생현저적간우작용。
A laboratory experiment was performed to evaluate the toxicological effects of endosulfan on some enzymes(urease, dehydroge-nase andβ-glucoside)and microbial community structure diversity in a brunisolic soil at different concentrations(0, 0.1, 1 mg and 10 mg endosulfan per kg soil)on the 7th, 14th, 21st and 28th day of treatment. Endosulfan did not influence soil urease activity, but significantly in-hibited dehydrogenase with stronger suppression at higher concentrations. However, the activity ofβ-glucoside enzyme was steadily and sig-nificantly enhanced by endosulfan. The DGGE data showed that endosulfan at 0~10 mg·kg-1 had no toxic effects on soil bacterial community structure and activities. In conclusion, endosulfan did not impact bacterial community structure in the brunisolic soil.