农业环境科学学报
農業環境科學學報
농업배경과학학보
Journal of Agro-Environment Science
2014年
11期
2125-2130
,共6页
张国福%李本杰%王金花%夏晓明
張國福%李本傑%王金花%夏曉明
장국복%리본걸%왕금화%하효명
苯醚甲环唑%嘧菌酯%剂型%斑马鱼%急性毒性
苯醚甲環唑%嘧菌酯%劑型%斑馬魚%急性毒性
분미갑배서%밀균지%제형%반마어%급성독성
difenoconazole%azoxystrobin%formulation%zebrafish%acute toxicity
为评价不同剂型农药对水生生物的毒性影响,采用半静态试验法,测定了不同剂型苯醚甲环唑和嘧菌酯及其原药对斑马鱼的急性毒性效应。结果表明,95%苯醚甲环唑原药、3%苯醚甲环唑悬浮种衣剂、400 g·L-1苯醚甲环唑悬浮剂和60%苯醚甲环唑水分散粒剂对斑马鱼96 h的LC50值与95%置信限(括号内)分别为1.05(0.93~1.13)、1.34(1.26~1.39)、1.44(1.37~1.52)、2.72(2.68~2.76)a.i.mg·L-1;93%嘧菌酯原药、10%嘧菌酯悬浮种衣剂、25%嘧菌酯悬浮剂和50%嘧菌酯水分散粒剂对斑马鱼96 h的LC50值与95%置信限(括号内)分别为0.67(0.64~0.72)、0.88(0.85~0.92)、1.03(0.98~1.07)、1.60(1.10~1.81)a.i.mg·L-1。依据《化学农药环境安全评价试验准则》,不同剂型苯醚甲环唑及原药对斑马鱼的急性毒性级别均为中毒;25%嘧菌酯悬浮剂和50%嘧菌酯水分散粒剂对斑马鱼的急性毒性级别为中毒;93%嘧菌酯原药和10%嘧菌酯悬浮种衣剂对斑马鱼的急性毒性级别为高毒。上述结果表明,不同剂型苯醚甲环唑和嘧菌酯及其原药对斑马鱼的急性毒性存在差异,毒性从高到低依次为:原药、悬浮种衣剂、悬浮剂、水分散粒剂。
為評價不同劑型農藥對水生生物的毒性影響,採用半靜態試驗法,測定瞭不同劑型苯醚甲環唑和嘧菌酯及其原藥對斑馬魚的急性毒性效應。結果錶明,95%苯醚甲環唑原藥、3%苯醚甲環唑懸浮種衣劑、400 g·L-1苯醚甲環唑懸浮劑和60%苯醚甲環唑水分散粒劑對斑馬魚96 h的LC50值與95%置信限(括號內)分彆為1.05(0.93~1.13)、1.34(1.26~1.39)、1.44(1.37~1.52)、2.72(2.68~2.76)a.i.mg·L-1;93%嘧菌酯原藥、10%嘧菌酯懸浮種衣劑、25%嘧菌酯懸浮劑和50%嘧菌酯水分散粒劑對斑馬魚96 h的LC50值與95%置信限(括號內)分彆為0.67(0.64~0.72)、0.88(0.85~0.92)、1.03(0.98~1.07)、1.60(1.10~1.81)a.i.mg·L-1。依據《化學農藥環境安全評價試驗準則》,不同劑型苯醚甲環唑及原藥對斑馬魚的急性毒性級彆均為中毒;25%嘧菌酯懸浮劑和50%嘧菌酯水分散粒劑對斑馬魚的急性毒性級彆為中毒;93%嘧菌酯原藥和10%嘧菌酯懸浮種衣劑對斑馬魚的急性毒性級彆為高毒。上述結果錶明,不同劑型苯醚甲環唑和嘧菌酯及其原藥對斑馬魚的急性毒性存在差異,毒性從高到低依次為:原藥、懸浮種衣劑、懸浮劑、水分散粒劑。
위평개불동제형농약대수생생물적독성영향,채용반정태시험법,측정료불동제형분미갑배서화밀균지급기원약대반마어적급성독성효응。결과표명,95%분미갑배서원약、3%분미갑배서현부충의제、400 g·L-1분미갑배서현부제화60%분미갑배서수분산립제대반마어96 h적LC50치여95%치신한(괄호내)분별위1.05(0.93~1.13)、1.34(1.26~1.39)、1.44(1.37~1.52)、2.72(2.68~2.76)a.i.mg·L-1;93%밀균지원약、10%밀균지현부충의제、25%밀균지현부제화50%밀균지수분산립제대반마어96 h적LC50치여95%치신한(괄호내)분별위0.67(0.64~0.72)、0.88(0.85~0.92)、1.03(0.98~1.07)、1.60(1.10~1.81)a.i.mg·L-1。의거《화학농약배경안전평개시험준칙》,불동제형분미갑배서급원약대반마어적급성독성급별균위중독;25%밀균지현부제화50%밀균지수분산립제대반마어적급성독성급별위중독;93%밀균지원약화10%밀균지현부충의제대반마어적급성독성급별위고독。상술결과표명,불동제형분미갑배서화밀균지급기원약대반마어적급성독성존재차이,독성종고도저의차위:원약、현부충의제、현부제、수분산립제。
Different formulations of pesticides show various toxicities to organisms. In this study, an acute toxicity experiment was conducted to evaluate the ecological risks of technical material and different formulations of difenoconazole and azoxystrobin to adult zebrafish using semi-static test. The 96 h LC50 values and 95%confidence limits for 95%difenoconazole technical material(TC), 3%difenoconazole flow-able concentrate for seed coating(FS), 400 g·L-1 difenoconazole aqueous suspension concentrate(SC), and 60%difenoconazole water dis-persible granule(WG)for adult zebrafish were 1.05 and 0.93~1.13, 1.34 and 1.26~1.39, 1.44 and 1.37~1.52, and 2.72 and 2.68~2.76 a.i.mg·L-1, respectively. Those of 93%azoxystrobin technical material(TC), 10%azoxystrobin flowable concentrate for seed coating(FS), 25%azoxystrobin aqueous suspension concentrate(SC), and 50%azoxystrobin water dispersible granule(WG)for adult zebrafish were re-spectively 0.67 and 0.64~0.72, 0.88 and 0.85~0.92, 1.03 and 0.98~1.07, and 1.60 and 1.10~1.81 a.i.mg·L-1. According to the Experimen-tal Guideline for Environmental Safety Evaluation of Chemical Pesticides, the toxicity of TC and different formulations of difenoconazole and that of 25%azoxystrobin SC and 50%azoxystrobin WG to adult zebrafish were moderate, while that of 93% azoxystrobin TC and 10%a-zoxystrobin FS was high. These results indicate that the toxicity of TC and different formulations of difenoconazole and azoxystrobin to adult zebrafish is different, being TC>FS>SC>WG.