国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2014年
11期
1956-1958
,共3页
缺氧诱导因子%Pax6%翼状胬肉
缺氧誘導因子%Pax6%翼狀胬肉
결양유도인자%Pax6%익상노육
hypoxia-inducible factor%Pax6%pterygium
目的：检测缺氧诱导因子( HIF-1α)和干细胞标志物( Pax6)在患者翼状胬肉组织中的表达,比较其与正常结膜组织中的变化,以探讨HIF-1α和Pax6的表达与翼状胬肉之间的关系。
<br> 方法：选取35例翼状胬肉患者切除的翼状胬肉组织为实验组,同时选取10例正常人结膜组织为对照组,使用常规免疫组织荧光染色法对HIF-1α和 Pax6的表达进行检测并与染色进行对照。
<br> 结果：实验组35例组织中HIF-1α的阳性表达率为66%(23/35),正常对照组10例组织中HIF-1α的阳性表达率仅为10%(1/10),HIF-1α表达在正常球结膜与翼状胬肉两组间的表达有统计学差异(P<0.05)。正常人球结膜Pax6在组织各层细胞核内表达,而Pax6在翼状胬肉组织中表达下降甚至未见表达,两组比较有统计学差异(P<0.05)。
<br> 结论：HIF-1α在翼状胬肉中高表达,提示它可能参与了翼状胬肉的病理过程；Pax6基因在翼状胬肉上皮细胞中表达下调,提示翼状胬肉上皮细胞病理过程属于鳞状上皮化生类型。
目的：檢測缺氧誘導因子( HIF-1α)和榦細胞標誌物( Pax6)在患者翼狀胬肉組織中的錶達,比較其與正常結膜組織中的變化,以探討HIF-1α和Pax6的錶達與翼狀胬肉之間的關繫。
<br> 方法：選取35例翼狀胬肉患者切除的翼狀胬肉組織為實驗組,同時選取10例正常人結膜組織為對照組,使用常規免疫組織熒光染色法對HIF-1α和 Pax6的錶達進行檢測併與染色進行對照。
<br> 結果：實驗組35例組織中HIF-1α的暘性錶達率為66%(23/35),正常對照組10例組織中HIF-1α的暘性錶達率僅為10%(1/10),HIF-1α錶達在正常毬結膜與翼狀胬肉兩組間的錶達有統計學差異(P<0.05)。正常人毬結膜Pax6在組織各層細胞覈內錶達,而Pax6在翼狀胬肉組織中錶達下降甚至未見錶達,兩組比較有統計學差異(P<0.05)。
<br> 結論：HIF-1α在翼狀胬肉中高錶達,提示它可能參與瞭翼狀胬肉的病理過程；Pax6基因在翼狀胬肉上皮細胞中錶達下調,提示翼狀胬肉上皮細胞病理過程屬于鱗狀上皮化生類型。
목적：검측결양유도인자( HIF-1α)화간세포표지물( Pax6)재환자익상노육조직중적표체,비교기여정상결막조직중적변화,이탐토HIF-1α화Pax6적표체여익상노육지간적관계。
<br> 방법：선취35례익상노육환자절제적익상노육조직위실험조,동시선취10례정상인결막조직위대조조,사용상규면역조직형광염색법대HIF-1α화 Pax6적표체진행검측병여염색진행대조。
<br> 결과：실험조35례조직중HIF-1α적양성표체솔위66%(23/35),정상대조조10례조직중HIF-1α적양성표체솔부위10%(1/10),HIF-1α표체재정상구결막여익상노육량조간적표체유통계학차이(P<0.05)。정상인구결막Pax6재조직각층세포핵내표체,이Pax6재익상노육조직중표체하강심지미견표체,량조비교유통계학차이(P<0.05)。
<br> 결론：HIF-1α재익상노육중고표체,제시타가능삼여료익상노육적병리과정；Pax6기인재익상노육상피세포중표체하조,제시익상노육상피세포병리과정속우린상상피화생류형。
AIM: To study the expression of hypoxia-inducible factor ( HIF ) and Pax6 in pterygium and investigate the relationship between expression of HIF and Pax6 in pterygium.
<br> METHODS:Thirty-five patients with pterygium who underwent simple excision and 10 cases of normal conjunctival tissues were enrolled in this study. Immunofluorescence was performed in pterygia tissue and normal conjunctiva, detecting the expression of HIF-1α and Pax6.
<br> RESULTS:The positive expression rate of HIF-1α was 66% ( 23/35 ) and 10% ( 1/10 ) in pterygia tissue and normal conjunctiva, respectively. The difference of expression between pterygia tissue and normal conjunctiva was statistically significant (P<0. 05). Expression of Pax6 was full-thickness nuclear in normal conjunctiva;however, expression of Pax6 decreased and even was negative in pterygium. The difference of expression in two groups was statistically significant (P<0. 05).
<br> CONCLUSION: HIF - 1α is highly expressed in pterygium, suggesting that it may be involved in the occurrence and development of pterygium. Pax6 gene downregulated in pterygium epithelial cells, suggesting that epithelial cells are squamous metaplasia.
