CAJ | 학술논문

目的：观察形觉剥夺性近视豚鼠视网膜组织学改变和TGF-β2在视网膜中的表达，为阐明TGF-β2在形觉剥夺性近视中的重要作用提供实验依据。 　　方法：用头套形觉剥夺右眼的方法诱导近视动物模型。实验前后检测豚鼠双眼屈光状态及眼轴长度。 HE染色后观察后极部视网膜形态学改变，通过免疫组化对TGF-β2在视网膜内表达进行定性及半定量测定。 　　结果：实验组视网膜变薄，且内核层的胞核层数较对照眼少，视网膜外核层细胞核变小、变圆，排列不均，视细胞层外节内节排列紊乱。免疫组化检查示实验组视网膜TGF-β2表达明显下调。 　　结论：头套遮盖效果明显，是一种诱导形觉剥夺性近视简便、稳定的方法。形觉剥夺性近视眼的视网膜产生了一系列的退行性改变。 TGF-β2可能是最终导致形觉剥夺性近视形成的重要因素之一。
목적：관찰형각박탈성근시돈서시망막조직학개변화TGF-β2재시망막중적표체，위천명TGF-β2재형각박탈성근시중적중요작용제공실험의거。 　　방법：용두투형각박탈우안적방법유도근시동물모형。실험전후검측돈서쌍안굴광상태급안축장도。 HE염색후관찰후겁부시망막형태학개변，통과면역조화대TGF-β2재시망막내표체진행정성급반정량측정。 　　결과：실험조시망막변박，차내핵층적포핵층수교대조안소，시망막외핵층세포핵변소、변원，배렬불균，시세포층외절내절배렬문란。면역조화검사시실험조시망막TGF-β2표체명현하조。 　　결론：두투차개효과명현，시일충유도형각박탈성근시간편、은정적방법。형각박탈성근시안적시망막산생료일계렬적퇴행성개변。 TGF-β2가능시최종도치형각박탈성근시형성적중요인소지일。
AIM: To observe the structure changes of retina and determine the expression of TGF-β2 in retina model. At the same time to clarify the function of TGF-β2 in form deprivation myopia model. METHODS: A facemask was worn on the right eyes of guinea pigs to develop form deprivation myopia. Before and after experiment, refraction was measured using retinoscope, and ocular axial was determined by A-scan ultrasonography. Morphologic alternations of the retina were observed by light microsope. The expression of TGF - β2 in the retina was determined by immunohistochemistry. RESULTS:Retina of myopic eyes became thinner, and inner nuclear layer of retina had few layers, nuclear of outer nuclear layer became smaller and circular, at the same time, they arranged in disorder. The immunohistochemisty results showed that expression of TGF-β2 in model eyes was lower than that in control eyes (P<0. 05). CONCLUSION: Covering eyes with facemasks is effective, simple and convenient in making myopic model. The retina of the model eye appeares degeneration. TGF-β2 plays an important role in the course of development in form deprivation myopia.