CAJ | 학술논문

目的：观察离焦诱导豚鼠近视眼视网膜超微结构特征及凋亡情况。 　　方法：将30只3周龄的三色豚鼠用-10.00 D硬性角膜接触镜( rigid gas permeable contact lens,RGP)对右眼进行离焦诱导,左眼不戴镜。3 wk后散瞳检影观察屈光度变化；电子透射显微镜观察视网膜超微结构特征；TUNEL免疫荧光染色检测视网膜细胞凋亡情况。 　　结果：右眼诱导前后屈光度差值为3.28±0.21D,左眼为1.55±0.23D,二者比较差异有显著统计学意义( P<0.01)；电镜结果显示,3wk后诱导眼视网膜可见膜盘结构的水肿,部分脱落；线粒体肿胀变形,部分空泡化；内外核层细胞膜不规则收缩,核染色质不规则聚集等凋亡特征；TUNEL免疫荧光染色显示诱导眼视网膜细胞凋亡发生率为(2.42±1.24)%,对照眼为(0.29±0.08)%,二者比较差异有显著统计学意义(P<0.01)。 　　结论：在离焦诱导的豚鼠近视眼的形成过程中伴随着视网膜细胞的异常凋亡。
목적：관찰리초유도돈서근시안시망막초미결구특정급조망정황。 　　방법：장30지3주령적삼색돈서용-10.00 D경성각막접촉경( rigid gas permeable contact lens,RGP)대우안진행리초유도,좌안불대경。3 wk후산동검영관찰굴광도변화；전자투사현미경관찰시망막초미결구특정；TUNEL면역형광염색검측시망막세포조망정황。 　　결과：우안유도전후굴광도차치위3.28±0.21D,좌안위1.55±0.23D,이자비교차이유현저통계학의의( P<0.01)；전경결과현시,3wk후유도안시망막가견막반결구적수종,부분탈락；선립체종창변형,부분공포화；내외핵층세포막불규칙수축,핵염색질불규칙취집등조망특정；TUNEL면역형광염색현시유도안시망막세포조망발생솔위(2.42±1.24)%,대조안위(0.29±0.08)%,이자비교차이유현저통계학의의(P<0.01)。 　　결론：재리초유도적돈서근시안적형성과정중반수착시망막세포적이상조망。
AIM: To observe retinal ultrastructural features and retinal cell apoptosis of defocus induced myopia in guinea pigs. METHODS: Thirty guinea pigs ( 3 - wk old ) were received -10. 00D rigid contact lenses (RGP) on the right eye and without wearing glasses on the left eye. Diopter change with cycloplegic retinoscopy, ultrastructural features of the retina with transmission electron microscopy were observed, the apoptosis of retinal cell by TUNEL immunofluorescence staining after 3wk were detected. RESULTS: Eye diopter difference was 3. 28±0. 21D in the right eye and 1. 55±0. 23D in the left eye before and after induction. There was significantly statistical difference (P<0. 01);Retinal ultrastructural features were showed with transmission electron microscopy ( TEM ) the retinal disk membranes edema, some shedding, mitochondrial swelling and deformation, small amount of vacuolar changes in the inner and outer nuclear layer cells. Some characteristics of apoptosis were also be seen such as cell membrane irregular contractions, irregular nuclear chromatin aggregation. The incidence of retinal cell apoptosis was (2. 42±1. 24)% in the right eye and ( 0. 29±0.08 )% in the left eye after induction. There was significantly statistical difference (P<0. 01).CONCLUSION:Accompanied by abnormal apoptosis of retinal cells during the formation of defocus-induced myopia in guinea pigs.