国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2014年
11期
1935-1938
,共4页
视网膜色素上皮细胞%吞噬负荷%感光细胞外节段%凋亡
視網膜色素上皮細胞%吞噬負荷%感光細胞外節段%凋亡
시망막색소상피세포%탄서부하%감광세포외절단%조망
retinal pigment epithelium%phagocytosic load%phagocytic outer segment%apoptosis
目的：了解吞噬负荷下体外培养老年牛视网膜色素上皮( retinal pigment epithelium,RPE)细胞的凋亡情况。
<br> 方法：三代体外培养老年牛RPE中分别加入培养液、含200μg/mL感光细胞外节段( photoreceptor outer segment, POS)的培养液、400μg/mL POS 培养液,分别于24,48h和96 h用流式细胞仪( AnnexinⅤ/PI双染法)检测细胞的凋亡水平。
<br> 结果：在各个时间点,空白对照组的RPE凋亡比例极低,200μg/mL POS组24 h凋亡比例(包括早期凋亡和晚期凋亡)为1.73%；随着时间延长,凋亡比例增加明显,到96 h时达到65.92%。而400μg/mL POS组24 h凋亡比例为4.02%,到96 h时则高达91.34%。
<br> 结论：吞噬负荷量的感光细胞外节段可以诱导体外培养老年牛RPE的凋亡,并且呈现剂量和时间依赖方式。
目的：瞭解吞噬負荷下體外培養老年牛視網膜色素上皮( retinal pigment epithelium,RPE)細胞的凋亡情況。
<br> 方法：三代體外培養老年牛RPE中分彆加入培養液、含200μg/mL感光細胞外節段( photoreceptor outer segment, POS)的培養液、400μg/mL POS 培養液,分彆于24,48h和96 h用流式細胞儀( AnnexinⅤ/PI雙染法)檢測細胞的凋亡水平。
<br> 結果：在各箇時間點,空白對照組的RPE凋亡比例極低,200μg/mL POS組24 h凋亡比例(包括早期凋亡和晚期凋亡)為1.73%；隨著時間延長,凋亡比例增加明顯,到96 h時達到65.92%。而400μg/mL POS組24 h凋亡比例為4.02%,到96 h時則高達91.34%。
<br> 結論：吞噬負荷量的感光細胞外節段可以誘導體外培養老年牛RPE的凋亡,併且呈現劑量和時間依賴方式。
목적：료해탄서부하하체외배양노년우시망막색소상피( retinal pigment epithelium,RPE)세포적조망정황。
<br> 방법：삼대체외배양노년우RPE중분별가입배양액、함200μg/mL감광세포외절단( photoreceptor outer segment, POS)적배양액、400μg/mL POS 배양액,분별우24,48h화96 h용류식세포의( AnnexinⅤ/PI쌍염법)검측세포적조망수평。
<br> 결과：재각개시간점,공백대조조적RPE조망비례겁저,200μg/mL POS조24 h조망비례(포괄조기조망화만기조망)위1.73%；수착시간연장,조망비례증가명현,도96 h시체도65.92%。이400μg/mL POS조24 h조망비례위4.02%,도96 h시칙고체91.34%。
<br> 결론：탄서부하량적감광세포외절단가이유도체외배양노년우RPE적조망,병차정현제량화시간의뢰방식。
AIM:To evaluate the effect of phagocytic load on apoptosis of cultured aged bovine retinal pigment epithelium ( RPE) cells.
<br> METHODS: The third generation of cultured aged bovine RPE cells was cultured respectively with culture medium, containing 200μg/mL photoreceptor outer segment ( POS) and 400μg/mL POS. Then the apoptosis rates of RPE cells after 24, 48 and 96h were detected with flow cytometry instrument ( AnnexinⅤ/PI double staining) .RESULTS: In blank control group, the apoptosis rates were very low at different time. After RPE cells were cultured with culture medium containing 200μg/mL POS for 24h, the apoptosis rate including early apoptosis and late apoptosis was 1. 73%. As time going, the apoptosis rate increased rapidly and reached 65. 92% after 96h. After RPE cells were cultured with culture medium containing 400μg/mL POS for 24h, the apoptosis rate was 4. 02%. And it reached 91. 34% significantly after 96h. CONCLUSION: Phagocytic loads can induce the apoptosis of RPE depending the time and dose.
