CAJ | 학술논문

采用脂肪酶Lipozyme RM IM催化辛酸、癸酸与大豆油进行酸解反应以制备MLM型结构脂质。通过单因素实验研究底物摩尔比、辛癸酸摩尔比、酶添加量、反应时间、反应温度和初始水分含量对酸解反应的影响。得到适合的反应条件为：底物摩尔比3∶1（总脂肪酸/大豆油），辛酸与癸酸摩尔比（辛酸/癸酸）2.5∶1，酶添加量7.5 wt%（基于底物总重），反应时间5 h，反应温度65℃，加水量1.0 wt%（基于底物总重），得到MLM结构脂脂肪酸组成中辛酸含量为20.0 wt%，癸酸含量为10.5 wt%，两者质量比为1.92。
채용지방매Lipozyme RM IM최화신산、계산여대두유진행산해반응이제비MLM형결구지질。통과단인소실험연구저물마이비、신계산마이비、매첨가량、반응시간、반응온도화초시수분함량대산해반응적영향。득도괄합적반응조건위：저물마이비3∶1（총지방산/대두유），신산여계산마이비（신산/계산）2.5∶1，매첨가량7.5 wt%（기우저물총중），반응시간5 h，반응온도65℃，가수량1.0 wt%（기우저물총중），득도MLM결구지지방산조성중신산함량위20.0 wt%，계산함량위10.5 wt%，량자질량비위1.92。
The acidic hydrolysis of soybean oil with caprylic acid and capric acid was utilized to manufacture MLM–type structured lipids using Lipozyme RM IM as the catalyst. Single factor experiments were applied to study the effects of substrates molar ratio,molar ratio of caprylic acid to capric acid,enzyme load,reaction time,reaction temperature and initial water content,giving the following optimum reaction conditions:substrate mole ratio 3∶1(total fatty acid/soybean oil),molar ratio of caprylic acid to capric acid 2.5∶1,enzyme load 7.5 wt%(of the substrate mass),5 h,65℃,and initial water content 1 wt%(of the substrate mass). The production contains caprylic acid 20.0 wt%, capric acid 10.5 wt%,and their mass ratio is 1.92.