CAJ | 학술논문

目的探讨大鼠骨髓间充质干细胞（BMSC）对颞下颌关节盘软骨细胞的作用。方法大鼠骨髓来源的间充质干细胞三向分化鉴定其干性，大鼠关节盘软骨细胞Ⅱ型胶原免疫荧光染色，收取BMSC培养24 h上清液制作条件培养基，CCK-8检测条件培养基对关节盘软骨细胞细胞增殖影响，反转录聚合酶链反应（RT-PCR）分析条件培养基培养7、14 d关节盘软骨Ⅰ、Ⅱ、Ⅹ型胶原基因表达。两组比较采用独立样本t检验，进行统计学分析。结果获取的大鼠BMSC具备三向分化能力，大鼠关节盘软骨细胞Ⅱ型胶原免疫荧光表达。 CCK-8结果显示，培养48 h 后条件培养基组细胞增殖速度明显加快，差异具有统计学意义（t=10.75，P<0.05）；RT-PCR结果显示：条件培养基组7 d时抑制Ⅹ型胶原表达（t=2.586，P<0.05），14 d时促进Ⅱ型胶原表达（t=6.501，P<0.05）。结论大鼠BMSC分泌物促进关节盘软骨细胞增殖，抑制关节盘软骨细胞肥大发挥保护作用。
목적탐토대서골수간충질간세포（BMSC）대섭하합관절반연골세포적작용。방법대서골수래원적간충질간세포삼향분화감정기간성，대서관절반연골세포Ⅱ형효원면역형광염색，수취BMSC배양24 h상청액제작조건배양기，CCK-8검측조건배양기대관절반연골세포세포증식영향，반전록취합매련반응（RT-PCR）분석조건배양기배양7、14 d관절반연골Ⅰ、Ⅱ、Ⅹ형효원기인표체。량조비교채용독립양본t검험，진행통계학분석。결과획취적대서BMSC구비삼향분화능력，대서관절반연골세포Ⅱ형효원면역형광표체。 CCK-8결과현시，배양48 h 후조건배양기조세포증식속도명현가쾌，차이구유통계학의의（t=10.75，P<0.05）；RT-PCR결과현시：조건배양기조7 d시억제Ⅹ형효원표체（t=2.586，P<0.05），14 d시촉진Ⅱ형효원표체（t=6.501，P<0.05）。결론대서BMSC분비물촉진관절반연골세포증식，억제관절반연골세포비대발휘보호작용。
Objective To study the role of rat bone marrow mesenchymal stem cells on disc cells from TMJ. Methods Culture rat bone marrow mesenchymal stem cells. These cells were induced into osteoblasts, chondrocytes, adipocytes to prove the potential of multilineage differentiation. The ColⅡof rat disc cells immunofluorescence assay in immunofluorescence microscopic. Prepared bone msenchyme stem cells conditioned medium (BMSC-CM) to culture rat TMJ disc cells. Cell Counting Kit-8 (CCK-8) analysis cell proliferation in conditioned medium. RT-PCR evaluated the changes in transcription genes of ColⅠ, ColⅡ, ColⅩ at 7 and 14 d. Results Rat BMSCs were successfully induced into osteoblasts, chondrocytes, adipocytes. ColⅡ immunofluorescence were detected in immunofluorescence microscopic. CCK-8 showed after culture 48 h, the disc cells proliferation increased condition medium compared with control group. RT-PCR revealed that ColⅠ, ColⅡ, ColⅩ were different between conditioned medium group and control group. Conclusion Rat BMSCs promote the disc cells proliferation and decrease the ColⅩ expression protect the disc cells.