CAJ | 학술논문

目的：观察通心络对高脂饲料联合小剂量链脲佐菌素( streptozotocin, STZ)诱导的早期糖尿病肾病( diabetic nephropathy, DN)大鼠血清一氧化氮( nitric oxid, NO)含量及内皮型一氧化氮合酶( eNOS)蛋白表达的影响,探讨通心络防治早期DN大鼠肾小球高滤过的作用机制。方法采用高脂饲料联合小剂量STZ建立DN大鼠模型,将DN大鼠随机分为模型组、缬沙坦组、通心络组各10只,设立10只正常SD大鼠为空白组,通心络组给予通心络超微粉0．4 g/( kg·d),缬沙坦组给予缬沙坦10 mg/( kg·d)干预8周,于治疗4周、8周监测空腹血糖( fasting blood glucose, FBG),尿微量白蛋白排泄率( urinary albumin excretion, UAER )、血肌酐( serum creatinine, SCr )及尿素氮( blood urea nitrogen, BUN),并计算内生肌酐清除率( creatinine clearance rate, Ccr)。于8周末处死大鼠,称量肾重,计算肾重指数,检测肾组织NO含量及eNOS蛋白表达。多组组间差异比较采用单因素方差分析。结果与空白组比较,模型组大鼠FBG、UAER、肾重及肾重指数、Scr、BUN、Ccr、NO含量显著升高、eNOS蛋白表达显著增强( P<0．05)。与模型组比较,通心络组及缬沙坦组FBG无明显变化(P>0．05),UAER、Scr、BUN、Ccr、肾重及肾重指数显著下降(P<0．05)。通心络组及缬沙坦组肾组织NO含量较模型组显著降低,eNOS蛋白表达较模型组减弱(P<0．05)。结论通心络可降低早期DN大鼠UAER、Scr、BUN、Ccr、肾重及肾重指数,保护肾功能。通心络抑制肾组织eNOS蛋白表达,减少肾组织NO的含量,从而降低早期DN大鼠肾小球率过滤,可能是通心络改善肾小球早期高滤过的作用机制之一。
목적：관찰통심락대고지사료연합소제량련뇨좌균소( streptozotocin, STZ)유도적조기당뇨병신병( diabetic nephropathy, DN)대서혈청일양화담( nitric oxid, NO)함량급내피형일양화담합매( eNOS)단백표체적영향,탐토통심락방치조기DN대서신소구고려과적작용궤제。방법채용고지사료연합소제량STZ건립DN대서모형,장DN대서수궤분위모형조、힐사탄조、통심락조각10지,설립10지정상SD대서위공백조,통심락조급여통심락초미분0．4 g/( kg·d),힐사탄조급여힐사탄10 mg/( kg·d)간예8주,우치료4주、8주감측공복혈당( fasting blood glucose, FBG),뇨미량백단백배설솔( urinary albumin excretion, UAER )、혈기항( serum creatinine, SCr )급뇨소담( blood urea nitrogen, BUN),병계산내생기항청제솔( creatinine clearance rate, Ccr)。우8주말처사대서,칭량신중,계산신중지수,검측신조직NO함량급eNOS단백표체。다조조간차이비교채용단인소방차분석。결과여공백조비교,모형조대서FBG、UAER、신중급신중지수、Scr、BUN、Ccr、NO함량현저승고、eNOS단백표체현저증강( P<0．05)。여모형조비교,통심락조급힐사탄조FBG무명현변화(P>0．05),UAER、Scr、BUN、Ccr、신중급신중지수현저하강(P<0．05)。통심락조급힐사탄조신조직NO함량교모형조현저강저,eNOS단백표체교모형조감약(P<0．05)。결론통심락가강저조기DN대서UAER、Scr、BUN、Ccr、신중급신중지수,보호신공능。통심락억제신조직eNOS단백표체,감소신조직NO적함량,종이강저조기DN대서신소구솔과려,가능시통심락개선신소구조기고려과적작용궤제지일。
Objective To investigate the influence of Tong Xinluo on renal function and the ex-pression of No, eNOS in kidney, and explore its mechanism of action. Methods The rat model was estab-lished by high fat feed and injection of streptozotocin. The model rats were separated into 3 groups random-ly:the model group(n=10), the valsartan group(n=10) and Tong Xinluo group(n=10). Another 10 normal rats were chosen as the normal group. Chinese herb medicine group was given Tong Xinluo 0. 4 g/( kg·d) and Western medicine group was given valsartan 10 mg/( kg·d) for 8 weeks. Fasting blood glu-cose(FBG), Urinary albumin excretion(UAER) , serum creatinine(Scr), blood urea nitrogen(BUN), Creatinine clearance rate( Ccr) were examined and calculated in 4weeks and 8weeks. After 8 weeks, all the rats were sacrificed, weighing their renal and observing the expression of NO, eNOS in kidney. Differ-ences among groups were assessed by one-way anova. Results Compared with normal group, the FBG, UAER , Scr , BUN, Ccr, kidney weight and relative kidney weight in model group are significantly in-creased(P<0. 05). Comperd with model group, the expression of FBG in valsartan group and Tong Xinluo group did not change(P>0. 05), while the UAER, Scr , BUN, Ccr, kidney weight and relative kidney weight significantly decreased(P<0. 05). The expression of NO, eNOS of TongXinluo group and valsartan group is also decreased(P<0. 05). Conclusion Tong Xinluo can decrease the expression of UAER, Scr , BUN, Ccr in DN rats, and then protect renal function. Decreasing the expression of NO and eNOS and then reducing glomerular hyperfiltration would be one of its action mechanism.