CAJ | 학술논문

目的：探讨COPD患者急性加重治疗前后IL-16、TNF-α和FIB在血浆水平的表达。方法收集2012年10月-2013年12月确诊为COPD(共96例)急性加重的患者,同时选取20例健康体检者为正常对照组。将受试者分两组,正常对照组(20例)和COPD(96例),并按肺功能分级将COPD组分为4组(Ι级20例,Ⅱ级26例,Ⅲ级23例,Ⅳ级27例,共96例),采用酶联免疫吸附法( ELASA)检测血清中IL-16、TNF-α和FIB的表达。结果 COPD患者血浆IL-16、TNF-α和 FIB水平显著高于健康对照组,P均<0．01。 COPD Ⅰ级、Ⅱ级、Ⅲ级、Ⅳ级两两比较,IL-16、TNF-α和 FIB表达水平随气道受限严重程度增高而显著升高,差异均有统计学意义(P 均<0．05)。血浆IL-16、TNF-α和FIB在COPD患者中呈直线正相关,相关系数分别为0．733、0．651和0．576,差异均具有统计学意义(P均<0．05)。 COPD急性加重患者血浆中IL-16、TNF-α、FIB水平在治疗后显著降低,但仍显著高于正常对照组( P均<0．01)。结论血浆 IL-16、TNF-α、 FIB在COPD中表达水平增高,并与肺功能下降程度呈正相关。
목적：탐토COPD환자급성가중치료전후IL-16、TNF-α화FIB재혈장수평적표체。방법수집2012년10월-2013년12월학진위COPD(공96례)급성가중적환자,동시선취20례건강체검자위정상대조조。장수시자분량조,정상대조조(20례)화COPD(96례),병안폐공능분급장COPD조분위4조(Ι급20례,Ⅱ급26례,Ⅲ급23례,Ⅳ급27례,공96례),채용매련면역흡부법( ELASA)검측혈청중IL-16、TNF-α화FIB적표체。결과 COPD환자혈장IL-16、TNF-α화 FIB수평현저고우건강대조조,P균<0．01。 COPD Ⅰ급、Ⅱ급、Ⅲ급、Ⅳ급량량비교,IL-16、TNF-α화 FIB표체수평수기도수한엄중정도증고이현저승고,차이균유통계학의의(P 균<0．05)。혈장IL-16、TNF-α화FIB재COPD환자중정직선정상관,상관계수분별위0．733、0．651화0．576,차이균구유통계학의의(P균<0．05)。 COPD급성가중환자혈장중IL-16、TNF-α、FIB수평재치료후현저강저,단잉현저고우정상대조조( P균<0．01)。결론혈장 IL-16、TNF-α、 FIB재COPD중표체수평증고,병여폐공능하강정도정정상관。
Objective To investigate the serum levels of IL-16, TNF-α and FIB in patients with COPD. Methods 96 patients with COPD were enrolled from August 2012 to October 2013, and 20 healthy subjects were se-lected as the control group. The subjects were divided into the normal control group (20 cases) and the COPD group. At the same time, all the COPD patients were divided into 4 groups by the classification of their lung function. The expression of IL-16, TNF-α and FIB in peripheral cytoplasm were detected by the method of double antibody sand-wich enzyme-linked immunosorbent assay (ELASA). Results In patients with COPD, IL-16, TNF-αand FIB lev-els in plasma were significantly higher than in the healthy control group (P<0. 01). In the COPD group, the expres-sion levels of IL-16, TNF-α and FIB increased significantly along with the airway limitation (P<0. 05). In patients with COPD, IL-16, TNF-αand FIB in plasma showed a positive linear correlation, with the correlation coefficient of 0. 733, 0. 651 and 0. 576 respectively (P<0. 05). In the patients with COPD, the plasma levels of IL-16, TNF-αand FIB reduced significantly after the treatment, but they were still significantly higher than in the control group ( P<0. 01). Conclusion The expression levels of IL-16, TNF-αand FIB increases in plasma of COPD paitients, and they are positively correlated with obstructive airflow limitation.