CAJ | 학술논문

目的：对急性心肌梗死（AMI）患者白介素-10（IL-10）基因启动子-592C/A进行多态性分析，探讨AMI患者IL-10基因-592C/A单核苷酸多态性分布及其与AMI发病的相关性。方法采用DNA试剂盒提取170例AMI患者（AMI组）及153例排除冠心病患者（对照组）的外周静脉血DNA，经聚合酶链反应（PCR）扩增目的DNA片段后，用试剂盒回收PCR产物，测序鉴定IL-10基因启动子-592C/A多态性，并检测血糖和血脂等生化指标的变化。结果 IL-10基因启动子-592C/A在AMI组和对照组中均存在CC、AC和AA 3种基因型；两组患者3种基因型分布频率及A、C等位基因频率比较，差异均无统计学意义（P＞0.05）；经对多个危险因素的多元logistic回归分析显示，IL-10基因启动子-592C/A 3种基因型与AMI的发病不相关。结论 IL-10基因启动子-592C/A多态性可能与AMI的发病无明显关系。
목적：대급성심기경사（AMI）환자백개소-10（IL-10）기인계동자-592C/A진행다태성분석，탐토AMI환자IL-10기인-592C/A단핵감산다태성분포급기여AMI발병적상관성。방법채용DNA시제합제취170례AMI환자（AMI조）급153례배제관심병환자（대조조）적외주정맥혈DNA，경취합매련반응（PCR）확증목적DNA편단후，용시제합회수PCR산물，측서감정IL-10기인계동자-592C/A다태성，병검측혈당화혈지등생화지표적변화。결과 IL-10기인계동자-592C/A재AMI조화대조조중균존재CC、AC화AA 3충기인형；량조환자3충기인형분포빈솔급A、C등위기인빈솔비교，차이균무통계학의의（P＞0.05）；경대다개위험인소적다원logistic회귀분석현시，IL-10기인계동자-592C/A 3충기인형여AMI적발병불상관。결론 IL-10기인계동자-592C/A다태성가능여AMI적발병무명현관계。
Objective To explore analyze the polymorphism of interleukin-10(IL-10)gene-592C/A of patients with acute myocardial infarction(AMI) and its relevance to the occurrence of AMI by analyzing the polymorphism of IL-10 gene promoter-592C/A. Methods DNA kit was adopted to extract the DNA from peripheral venous blood of 170 AMI patients(AMI group) and 153 controls (control group) at the exclusion of coronary heart disease (CHD),after amplifying DNA segments by polymerases chain reaction(PCR),the kit was used to recycle reaction products of PCR to identify the polymorphism of IL-10 gene-529C/A and detect the biochemical indicators including blood glucose and blood lipid. Results IL-10 gene-592C/A had 3 genotypes(CC, AC and AA) in both AMI group and control group;there was no statistically significant difference on distribution frequencies of the 3 genotypes and allele frequencies of A and C between the AMI group and control group (P>0.05);the results of multivariate logis-tic regression analysis in multiple risk factors showed that the genotypes of IL-gene-592C/A were not revelent to the occurrence of AMI. Conclusion The polymorphism of IL-10 gene-592C/A has no relationship with AMI in the crowds of Changzhou region.